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Hofmeister challenges: Ion binding and charge of the BSA protein as explicit examples
KTH, School of Industrial Engineering and Management (ITM), Materials Science and Engineering.
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2012 (English)In: Langmuir, ISSN 0743-7463, E-ISSN 1520-5827, Vol. 28, no 47, 16355-16363 p.Article in journal (Refereed) Published
Abstract [en]

Experiments on bovine serum albumin (BSA) via potentiometric titration (PT) and electrophoretic light scattering (ELS) are used to study specific-ion binding. The effect is appreciable at a physiological concentration of 0.1 M. We found that anions bind to the protein surface at an acidic pH, where the protein carries a positive charge (Z p > 0), according to a Hofmeister series (Cl - < Br - < NO 3 - < I - < SCN -), as well as at the isoionic point (Z p = 0). The results obtained require critical interpretation. The measurements performed depend on electrostatic theories that ignore the very specific effects they are supposed to reveal. Notwithstanding this difficulty, we can still infer that different 1:1 sodium salts affect the BSA surface charge/pH curve because anions bind to the BSA surface with an efficiency which follows a Hofmeister series.

Place, publisher, year, edition, pages
2012. Vol. 28, no 47, 16355-16363 p.
Keyword [en]
acidic ph, bovine serum albumins, electrophoretic light scattering, electrostatic theories, hofmeister series, ion binding, physiological concentrations, positive charges, potentiometric titrations, protein surface, sodium salt, specific effects
National Category
Engineering and Technology
Identifiers
URN: urn:nbn:se:kth:diva-107990DOI: 10.1021/la3035984ISI: 000312515200015Scopus ID: 2-s2.0-84870170136OAI: oai:DiVA.org:kth-107990DiVA: diva2:578853
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QC 20121219

Available from: 2012-12-19 Created: 2012-12-19 Last updated: 2017-12-06Bibliographically approved

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