A Noncanonical Postsynaptic Transport Route for a GPCR Belonging to the Serotonin Receptor Family
2012 (English)In: Journal of Neuroscience, ISSN 0270-6474, E-ISSN 1529-2401, Vol. 32, no 50, 17998-18008 p.Article in journal (Refereed) Published
Postsynaptic receptor trafficking plays an essential role in tuning neurotransmission and signal plasticity and has emerged as a potential therapeutic target in neuropsychiatric disease. Using a novel application of fluorescence recovery after photobleaching in rat hippocampal neurons, we examined transport from the soma to dendrites of seven G-protein-coupled receptors (GPCRs) implicated in mood disorders. Most GPCRs were delivered to dendrites via lateral diffusion, but one GPCR, the serotonin 1B receptor (5-HT1B), was delivered to the dendrites in secretory vesicles. Within the dendrites, 5-HT1B were stored in a reservoir of accessible vesicles that were recruited to preferential sites in plasma membrane, as observed with superecliptic pHluorin labeling. After membrane recruitment, 5-HT1B transport via lateral diffusion and temporal confinement to inhibitory and excitatory synapses was monitored by single particle tracking. These results suggest an alternative mechanism for control of neuronal activity via a GPCR that has been implicated in mood regulation.
Place, publisher, year, edition, pages
2012. Vol. 32, no 50, 17998-18008 p.
Mu-Opioid Receptors, Dopamine D1 Receptors, Synaptic-Transmission, Diffusion Dynamics, Lateral Mobility, Plasma-Membrane, 5-Ht1b Receptor, Ampa Receptors, Quantum Dots, Trafficking
IdentifiersURN: urn:nbn:se:kth:diva-110060DOI: 10.1523/JNEUROSCI.1804-12.2012ISI: 000312404700008ScopusID: 2-s2.0-84871044414OAI: oai:DiVA.org:kth-110060DiVA: diva2:585667
FunderSwedish Research Council, 2007-4582 2010-3261Science for Life Laboratory - a national resource center for high-throughput molecular bioscience
QC 201301102013-01-102013-01-102013-01-10Bibliographically approved