Change search
ReferencesLink to record
Permanent link

Direct link
Estimating Microtubule Distributions from 2D Immunofluorescence Microscopy Images Reveals Differences among Human Cultured Cell Lines
KTH, School of Biotechnology (BIO), Proteomics (closed 20130101). KTH, Centres, Science for Life Laboratory, SciLifeLab.
KTH, School of Biotechnology (BIO), Proteomics (closed 20130101). KTH, Centres, Science for Life Laboratory, SciLifeLab.ORCID iD: 0000-0001-7034-0850
Show others and affiliations
2012 (English)In: PLoS ONE, ISSN 1932-6203, Vol. 7, no 11, e50292- p.Article in journal (Refereed) Published
Abstract [en]

Microtubules are filamentous structures that are involved in several important cellular processes, including cell division, cellular structure and mechanics, and intracellular transportation. Little is known about potential differences in microtubule distributions within and across cell lines. Here we describe a method to estimate information pertaining to 3D microtubule distributions from 2D fluorescence images. Our method allows for quantitative comparisons of microtubule distribution parameters (number of microtubules, mean length) between different cell lines. Among eleven cell lines compared, some showed differences that could be accounted for by differences in the total amount of tubulin per cell while others showed statistically significant differences in the balance between number and length of microtubules. We also observed that some cell lines that visually appear different in their microtubule distributions are quite similar when the model parameters are considered. The method is expected to be generally useful for comparing microtubule distributions between cell lines and for a given cell line after various perturbations. The results are also expected to enable analysis of the differences in gene expression underlying the observed differences in microtubule distributions among cell types.

Place, publisher, year, edition, pages
2012. Vol. 7, no 11, e50292- p.
Keyword [en]
article, cell shape, cell type, cellular distribution, computer simulation, fluorescence imaging, gene expression, human, human cell, human cell culture, image analysis, immunofluorescence microscopy, microtubule assembly, molecular imaging, molecular model, quantitative analysis, three dimensional imaging
National Category
Cell and Molecular Biology
URN: urn:nbn:se:kth:diva-110210DOI: 10.1371/journal.pone.0050292ISI: 000312601700052ScopusID: 2-s2.0-84870341521OAI: diva2:586126
Science for Life Laboratory - a national resource center for high-throughput molecular bioscienceKnut and Alice Wallenberg Foundation

QC 20130111

Available from: 2013-01-11 Created: 2013-01-10 Last updated: 2013-01-21Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textScopus

Search in DiVA

By author/editor
Wiking, MikaelaLundberg, Emma
By organisation
Proteomics (closed 20130101)Science for Life Laboratory, SciLifeLab
In the same journal
Cell and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 26 hits
ReferencesLink to record
Permanent link

Direct link