Ultra-localized single cell electroporation using silicon nanowires
2013 (English)In: Lab on a Chip, ISSN 1473-0197, E-ISSN 1473-0189, Vol. 13, no 3, 336-339 p.Article in journal (Refereed) Published
Analysis of cell-to-cell variation can further the understanding of intracellular processes and the role of individual cell function within a larger cell population. The ability to precisely lyse single cells can be used to release cellular components to resolve cellular heterogeneity that might be obscured when whole populations are examined. We report a method to position and lyse individual cells on silicon nanowire and nanoribbon biological field effect transistors. In this study, HT-29 cancer cells were positioned on top of transistors by manipulating magnetic beads using external magnetic fields. Ultra-rapid cell lysis was subsequently performed by applying 600-900 mV(pp) at 10 MHz for as little as 2 ms across the transistor channel and the bulk substrate. We show that the fringing electric field at the device surface disrupts the cell membrane, leading to lysis from irreversible electroporation. This methodology allows rapid and simple single cell lysis and analysis with potential applications in medical diagnostics, proteome analysis and developmental biology studies.
Place, publisher, year, edition, pages
2013. Vol. 13, no 3, 336-339 p.
Microfluidic Devices, Lysis, Manipulation, Potentials, Chip
Biochemistry and Molecular Biology
IdentifiersURN: urn:nbn:se:kth:diva-117655DOI: 10.1039/c2lc40837fISI: 000312947300003ScopusID: 2-s2.0-84872085096OAI: oai:DiVA.org:kth-117655DiVA: diva2:602809
QC 201302042013-02-042013-02-012013-11-11Bibliographically approved