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Early Cone Setting in Picea abies acrocona Is Associated with Increased Transcriptional Activity of a MADS Box Transcription Factor
KTH, School of Biotechnology (BIO), Gene Technology. KTH, Centres, Science for Life Laboratory, SciLifeLab.
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2013 (English)In: Plant Physiology, ISSN 0032-0889, E-ISSN 1532-2548, Vol. 161, no 2, 813-823 p.Article in journal (Refereed) Published
Abstract [en]

Conifers normally go through a long juvenile period, for Norway spruce (Picea abies) around 20 to 25 years, before developing male and female cones. We have grown plants from inbred crosses of a naturally occurring spruce mutant (acrocona). One-fourth of the segregating acrocona plants initiate cones already in their second growth cycle, suggesting control by a single locus. The early cone-setting properties of the acrocona mutant were utilized to identify candidate genes involved in vegetative-to-reproductive phase change in Norway spruce. Poly(A(+)) RNA samples from apical and basal shoots of cone-setting and non-cone-setting plants were subjected to high-throughput sequencing (RNA-seq). We assembled and investigated 33,383 expressed putative protein-coding acrocona transcripts. Eight transcripts were differentially expressed between selected sample pairs. One of these (Acr42124_1) was significantly up-regulated in apical shoot samples from cone-setting acrocona plants, and the encoded protein belongs to the MADS box gene family of transcription factors. Using quantitative real-time polymerase chain reaction with independently derived plant material, we confirmed that the MADS box gene is up-regulated in both needles and buds of cone-inducing shoots when reproductive identity is determined. Our results constitute important steps for the development of a rapid cycling model system that can be used to study gene function in conifers. In addition, our data suggest the involvement of a MADS box transcription factor in the vegetative-to-reproductive phase change in Norway spruce.

Place, publisher, year, edition, pages
2013. Vol. 161, no 2, 813-823 p.
Keyword [en]
Norway Spruce, Rna-Seq, Gene Family, Arabidopsis, Plants, Growth, Trees, Diversification, Identification, Information
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:kth:diva-119047DOI: 10.1104/pp.112.207746ISI: 000314360100018Scopus ID: 2-s2.0-84873256547OAI: oai:DiVA.org:kth-119047DiVA: diva2:609405
Funder
Swedish Research CouncilScience for Life Laboratory - a national resource center for high-throughput molecular bioscience
Note

QC 20130305

Available from: 2013-03-05 Created: 2013-03-05 Last updated: 2017-12-06Bibliographically approved

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Emanuelsson, Olof

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