Change search
ReferencesLink to record
Permanent link

Direct link
An automated approach for three-dimensional quantification of fibrillar structures in optically cleared soft biological tissues
Show others and affiliations
2013 (English)In: Journal of the Royal Society Interface, ISSN 1742-5662, E-ISSN 1742-5689, Vol. 10, no 80, 20120760- p.Article in journal (Refereed) Published
Abstract [en]

We present a novel approach allowing for a simple, fast and automated morphological analysis of three-dimensional image stacks (z-stacks) featuring fibrillar structures from optically cleared soft biological tissues. Five non-atherosclerotic tissue samples from human abdominal aortas were used to outline the multi-purpose methodology, applicable to various tissue types. It yields a three-dimensional orientational distribution of relative amplitudes, representing the original collagen fibre morphology, identifies regions of isotropy where no preferred fibre orientations are observed and determines structural parameters throughout anisotropic regions for the analysis and numerical modelling of biomechanical quantities such as stress and strain. Our method combines optical tissue clearing with second-harmonic generation imaging, Fourier-based image analysis and maximum-likelihood estimation for distribution fitting. With a new sample preparation method for arteries, we present, for the first time to our knowledge, a continuous three-dimensional distribution of collagen fibres throughout the entire thickness of the aortic wall, revealing novel structural and organizational insights into the three arterial layers.

Place, publisher, year, edition, pages
2013. Vol. 10, no 80, 20120760- p.
Keyword [en]
collagen, artery, constitutive modelling, fibre orientation, optical clearing, second-harmonic generation
National Category
Engineering and Technology
URN: urn:nbn:se:kth:diva-119032DOI: 10.1098/rsif.2012.0760ISI: 000314285400005ScopusID: 2-s2.0-84873620473OAI: diva2:609952

QC 20130308

Available from: 2013-03-08 Created: 2013-03-05 Last updated: 2013-03-08Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textScopus

Search in DiVA

By author/editor
Holzapfel, Gerhard A.
By organisation
Solid Mechanics (Dept.)
In the same journal
Journal of the Royal Society Interface
Engineering and Technology

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 21 hits
ReferencesLink to record
Permanent link

Direct link