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An automated approach for three-dimensional quantification of fibrillar structures in optically cleared soft biological tissues
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2013 (English)In: Journal of the Royal Society Interface, ISSN 1742-5689, E-ISSN 1742-5662, Vol. 10, no 80, 20120760- p.Article in journal (Refereed) Published
Abstract [en]

We present a novel approach allowing for a simple, fast and automated morphological analysis of three-dimensional image stacks (z-stacks) featuring fibrillar structures from optically cleared soft biological tissues. Five non-atherosclerotic tissue samples from human abdominal aortas were used to outline the multi-purpose methodology, applicable to various tissue types. It yields a three-dimensional orientational distribution of relative amplitudes, representing the original collagen fibre morphology, identifies regions of isotropy where no preferred fibre orientations are observed and determines structural parameters throughout anisotropic regions for the analysis and numerical modelling of biomechanical quantities such as stress and strain. Our method combines optical tissue clearing with second-harmonic generation imaging, Fourier-based image analysis and maximum-likelihood estimation for distribution fitting. With a new sample preparation method for arteries, we present, for the first time to our knowledge, a continuous three-dimensional distribution of collagen fibres throughout the entire thickness of the aortic wall, revealing novel structural and organizational insights into the three arterial layers.

Place, publisher, year, edition, pages
2013. Vol. 10, no 80, 20120760- p.
Keyword [en]
collagen, artery, constitutive modelling, fibre orientation, optical clearing, second-harmonic generation
National Category
Engineering and Technology
Identifiers
URN: urn:nbn:se:kth:diva-119032DOI: 10.1098/rsif.2012.0760ISI: 000314285400005Scopus ID: 2-s2.0-84873620473OAI: oai:DiVA.org:kth-119032DiVA: diva2:609952
Note

QC 20130308

Available from: 2013-03-08 Created: 2013-03-05 Last updated: 2017-12-06Bibliographically approved

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Holzapfel, Gerhard A.
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  • de-DE
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  • en-US
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  • nn-NB
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  • Other locale
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