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Lab-on-DVD: standard DVD drives as a novel laser scanning microscope for image based point of care diagnostics
KTH, School of Biotechnology (BIO), Proteomics (closed 20130101). KTH, Centres, Science for Life Laboratory, SciLifeLab.ORCID iD: 0000-0001-5199-0663
KTH, Centres, Science for Life Laboratory, SciLifeLab.
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2013 (English)In: Lab on a Chip, ISSN 1473-0197, E-ISSN 1473-0189, Vol. 13, no 8, 1578-1585 p.Article in journal (Refereed) Published
Abstract [en]

We present a novel "Lab-on-DVD" system and demonstrate its capability for rapid and low-cost HIV diagnostics by counting CD4+ cells isolated from whole blood. We show that a commercial DVD drive can, with certain modifications, be turned into an improved DVD-based laser scanning microscope (DVD-LSM). The system consists of a multi-layered disposable polymer disc and a modified commercial DVD reader with rotational control for sample handling, temperature control for optimized bioassay, a photodiode array for detection, and software for signal processing and user interface - all the necessary components required for a truly integrated lab-on-a-chip system, with the capability to deliver high-resolution images down to 1 mm in size. Using discs modified with antibodies, we specifically captured CD4+ cells from whole blood, demonstrating single cell resolution imaging. The novel integrated DVD platform with sub-micron image resolution brings, for the first time, affordable cellular diagnostic testing to the point-of-care and should be readily applicable at resource-limited settings.

Place, publisher, year, edition, pages
2013. Vol. 13, no 8, 1578-1585 p.
Keyword [en]
Platform, Microchip, Device
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:kth:diva-120566DOI: 10.1039/c3lc41360hISI: 000316275200019Scopus ID: 2-s2.0-84876710066OAI: oai:DiVA.org:kth-120566DiVA: diva2:615798
Funder
EU, FP7, Seventh Framework ProgrammeScience for Life Laboratory - a national resource center for high-throughput molecular bioscience
Note

QC 20130412

Available from: 2013-04-12 Created: 2013-04-11 Last updated: 2017-12-06Bibliographically approved

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Ramachandraiah, HarishaAmasia, MaryRussom, Aman
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