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Lab-on-DVD: standard DVD drives as a novel laser scanning microscope for image based point of care diagnostics
KTH, School of Biotechnology (BIO), Proteomics (closed 20130101). KTH, Centres, Science for Life Laboratory, SciLifeLab.
KTH, Centres, Science for Life Laboratory, SciLifeLab.
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2013 (English)In: Lab on a Chip, ISSN 1473-0197, E-ISSN 1473-0189, Vol. 13, no 8, 1578-1585 p.Article in journal (Refereed) Published
Abstract [en]

We present a novel "Lab-on-DVD" system and demonstrate its capability for rapid and low-cost HIV diagnostics by counting CD4+ cells isolated from whole blood. We show that a commercial DVD drive can, with certain modifications, be turned into an improved DVD-based laser scanning microscope (DVD-LSM). The system consists of a multi-layered disposable polymer disc and a modified commercial DVD reader with rotational control for sample handling, temperature control for optimized bioassay, a photodiode array for detection, and software for signal processing and user interface - all the necessary components required for a truly integrated lab-on-a-chip system, with the capability to deliver high-resolution images down to 1 mm in size. Using discs modified with antibodies, we specifically captured CD4+ cells from whole blood, demonstrating single cell resolution imaging. The novel integrated DVD platform with sub-micron image resolution brings, for the first time, affordable cellular diagnostic testing to the point-of-care and should be readily applicable at resource-limited settings.

Place, publisher, year, edition, pages
2013. Vol. 13, no 8, 1578-1585 p.
Keyword [en]
Platform, Microchip, Device
National Category
Biochemistry and Molecular Biology
URN: urn:nbn:se:kth:diva-120566DOI: 10.1039/c3lc41360hISI: 000316275200019ScopusID: 2-s2.0-84876710066OAI: diva2:615798
EU, FP7, Seventh Framework ProgrammeScience for Life Laboratory - a national resource center for high-throughput molecular bioscience

QC 20130412

Available from: 2013-04-12 Created: 2013-04-11 Last updated: 2013-04-12Bibliographically approved

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Ramachandraiah, HarishaAmasia, MaryRussom, Aman
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Proteomics (closed 20130101)Science for Life Laboratory, SciLifeLab
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