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Sustainable bio-production of styrene from forest waste
KTH, School of Chemical Science and Engineering (CHE), Chemistry, Organic Chemistry. (Ecological Chemistry Group)
KTH, School of Chemical Science and Engineering (CHE), Chemistry, Organic Chemistry. (Ecological Chemistry Group)
KTH, School of Biotechnology (BIO), Industrial Biotechnology.
2013 (English)In: Bioresource Technology, ISSN 0960-8524, E-ISSN 1873-2976, Vol. 144, 684-688 p.Article in journal (Refereed) Published
Abstract [en]

A strain of Penicillium expansum was studied for the production of styrene using forest waste biomass as a feeding substrate. The fungal strain was cultivated on bark of various trees supplemented with yeast extract and the volatiles produced were collected on Tenax TA and analyzed by gas chromatography-mass spectrometry. Fungus cultured on grated soft bark of pine (Pinus sylvestris) stems (GPB) and mature bark of oak (Quercus robur) supplemented with yeast extract produced relatively the highest amounts of styrene. The maximum styrene production rate was 52.5 mu g/h, 41 mu g/h and 27 mu g/h from fungus cultivated on 50 mL liquid media with 10 g GPB or mature bark of oak and potato dextrose broth respectively. These promising results suggest that the fungal strain could be used to produce "green" styrene plastics using renewable forest waste biomass.

Place, publisher, year, edition, pages
2013. Vol. 144, 684-688 p.
Keyword [en]
Forest biomass, Penicillium expansum, Styrene, Bark, Fungal volatiles
National Category
Other Chemistry Topics Energy Engineering
URN: urn:nbn:se:kth:diva-121994DOI: 10.1016/j.biortech.2013.07.033ISI: 000324280100093ScopusID: 2-s2.0-84881558867OAI: diva2:619972

QC 20131017. Updated from submitted to published.

Available from: 2013-05-07 Created: 2013-05-07 Last updated: 2013-10-17Bibliographically approved
In thesis
1. Microbes Associated with Hylobius abietis: A Chemical and Behavioral Study
Open this publication in new window or tab >>Microbes Associated with Hylobius abietis: A Chemical and Behavioral Study
2013 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

This thesis is based on three inter-related studies: the first part deals with the microbial consortium, the identification of microbes and their volatiles, the second part deals with the study of bio-chemical control methods of two conifer pests; the pine weevil Hylobius abietis (L.) and the root rot fungi Heterobasidion spp., and the third part describes the production of styrene by a fungus using forest waste.The large pine weevil (Hylobius abietis L.) is an economically important pest insect of conifers in reforestation areas of Europe and Asia. The female weevils protect their eggs from feeding conspecifics by adding frass (mixture of weevil feces and chewed bark) along with the eggs. In order to understand the mechanism behind frass deposition at the egg laying site and to find repellents/antifeedants for pine weevils, microbes were isolated from the aseptically collected pine weevil frass. Microbial produced volatile organic compounds (VOCs) were collected by solid phase micro extraction and analyzed by GC-MS after cultivating them on weevil frass broth. The major VOCs were tested against pine weevils using a multi-choice olfactometer. Ewingella sp., Mucor racemosus, Penicillium solitum, P. expansum, Ophiostoma piceae, O. pluriannulatum, Debaryomyces hansenii and Candida sequanensis were identified as abundant microbes. Styrene, 6-protoilludene, 1-octene-3-ol, 3-methylanisole, methyl salicylate, 2-methoxyphenol and 2-methoxy-4-vinylphenol were the VOCs of persistently isolated microbes. In behavioral bioassay, methyl salicylate, 3-methylanisole and styrene significantly reduced the attraction of pine weevils to their host plant volatiles. Heterobasidion spp. are severe pathogenic fungi of conifers that cause root and butt rot in plants. Bacterial isolates were tested for the antagonistic activity against fungi on potato dextrose agar. Bacillus subtilis strains significantly inhibited the growth of H. annosum and H. parviporum. Styrene is an industrial chemical used for making polymeric products, currently produced from fossil fuel. A strain of Penicillium expansum isolated from pine weevil frass was investigated for the production of styrene using forest waste. Grated pine stem bark and mature oak bark supplemented with yeast extract produced greater amounts of styrene compared to potato dextrose broth.

Place, publisher, year, edition, pages
Stockholm: KTH Royal Institute of Technology, 2013. 66 p.
Trita-CHE-Report, ISSN 1654-1081 ; 2013:23
Hylobius, Ewingella, Penicillium, Heterobasidion, Bacteria, Fungi, Bark, Forest waste, Metabolites, Styrene, Methyl salicylate.
National Category
Natural Sciences Organic Chemistry
urn:nbn:se:kth:diva-121887 (URN)978-91-7501-727-3 (ISBN)
Public defence
2013-05-30, F3, Lindstedtsvägen 26, KTH, Stockholm, 10:00 (English)

QC 20130507

Available from: 2013-05-07 Created: 2013-05-06 Last updated: 2013-05-07Bibliographically approved

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