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Interfacial cross-linking of beta-casein changes the structure of the adsorbed layer
KTH, School of Chemical Science and Engineering (CHE), Chemistry, Surface and Corrosion Science.ORCID iD: 0000-0001-7496-1101
2013 (English)In: Food Hydrocolloids, ISSN 0268-005X, Vol. 32, no 2, 271-277 p.Article in journal (Refereed) Published
Abstract [en]

The mechanism of transglutaminase-induced cross-linking of interfacial beta-casein layer was investigated in tetradecane/buffer system. Monolayer studies were carried out in a Langmuir trough, where incubation with the enzyme mostly affected the compression of the film through adsorption of transglutaminase to the interface. Interfacial shear rheology was used to follow the kinetics of formation of a visco-elastic film upon cross-linking. Substrate concentration affected the rate of the interfacial cross-linking, when enzyme was dosed per protein concentration. This was most likely due to the saturated substrate layer at the interface in all cases. SDS-PAGE revealed that most of the beta-casein at the interface was not cross-linked by intermolecular links, but rather, intramolecular links were formed. Finally, studies of adsorbed beta-casein layers on polystyrene beads revealed that cross-linking reduced the thickness of the adsorption layer from 11-12 nm to 8-9 nm. These results suggest that it may be mainly intra-molecular cross-linking which modifies the physical interactions of beta-caseins at the interface resulting in a higher layer density and thus, formation of a visco-elastic network.

Place, publisher, year, edition, pages
2013. Vol. 32, no 2, 271-277 p.
Keyword [en]
beta-Casein, Interface, Cross-linking, Rheology, Thickness
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Other Chemistry Topics
URN: urn:nbn:se:kth:diva-122316DOI: 10.1016/j.foodhyd.2013.01.009ISI: 000317184100008ScopusID: 2-s2.0-84874016803OAI: diva2:622030

QC 20130520

Available from: 2013-05-20 Created: 2013-05-20 Last updated: 2013-05-20Bibliographically approved

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Blomberg, Eva
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