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A family of small tyrosine rich proteins is essential for oogonial and oospore cell wall development of the mycoparasitic oomycete Pythium oligandrum
KTH, School of Biotechnology (BIO), Glycoscience.
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2013 (English)In: Fungal Biology, ISSN 1878-6146, E-ISSN 1878-6162, Vol. 117, no 3, 163-172 p.Article in journal (Refereed) Published
Abstract [en]

The mycoparasitic oomycete Pythium oligandrum is homothallic, producing an abundance of thick-walled spiny oospores in culture. After mining a cDNA sequence dataset, we identified a family of genes that code for small tyrosine rich (Pythium oligandrum small tyrosine rich (PoStr)) proteins. Sequence analysis identified similarity between the PoStr proteins and putative glycine-rich cell wall proteins from the related plant pathogenic oomycete Pythium ultimum, and mating-induced genes from the oomycete Phytophthora infestans. Expression analysis showed that PoStr transcripts accumulate during oospore production in culture and immunolocalisation indicates the presence of these proteins in oogonial and oospore cell walls. PoStr protein abundance correlated positively with production of oogonia as determined by antibiotic-mediated oogonia suppression. To further characterise the role of PoStr proteins in P. oligandrum oospore production, we silenced this gene family using homology-dependent gene silencing. This represents the first characterisation of genes using gene silencing in a Pythium species. Oospores from silenced strains displayed major ultrastructural changes and were sensitive to degradative enzyme treatment. Oogonia of silenced strains either appeared to be arrested at the mature oosphere stage of development or in around 40 % of the structures, showed a complete suppression of oospore formation. Suppressed oogonia were highly vacuolated and the oogonium wall was thickened by a new inner wall layer. Our data suggest PoStr proteins are probably integral structural components of the normal oospore cell wall and play a key role in oospore formation.

Place, publisher, year, edition, pages
2013. Vol. 117, no 3, 163-172 p.
Keyword [en]
Gene silencing, Mycoparasite, Oomycete, Oomycete sexual reproduction, Oospore, Oospore formation, Oosporogenesis, Pythium, TEM, Ultrastructure
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:kth:diva-122528DOI: 10.1016/j.funbio.2013.01.001ISI: 000317806400001Scopus ID: 2-s2.0-84875610682OAI: oai:DiVA.org:kth-122528DiVA: diva2:623083
Funder
EU, FP7, Seventh Framework Programme, FP7-PEOPLE-2010-IEF; CBOP
Note

QC 20130524

Available from: 2013-05-24 Created: 2013-05-23 Last updated: 2017-12-06Bibliographically approved

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