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Centrosome Isolation and Analysis by Mass Spectrometry-Based Proteomics
KTH, School of Biotechnology (BIO), Proteomics.ORCID iD: 0000-0001-7034-0850
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2013 (English)In: CILIA, PART B, Academic Press, 2013, 371-393 p.Chapter in book (Refereed)
Abstract [en]

Centrioles are microtubule-based scaffolds that are essential for the formation of centrosomes, cilia, and flagella with important functions throughout the cell cycle, in physiology and during development. The ability to purify centriole-containing organelles on a large scale, combined with advances in protein identification using mass spectrometry-based proteomics, have revealed multiple centriole-associated proteins that are conserved during evolution in eukaryotes. Despite these advances, the molecular basis for the plethora of processes coordinated by cilia and centrosomes is not fully understood. Considering the complexity and dynamics of centriole-related proteomes and the first-pass analyses reported so far, it is likely that further insight might come from more thorough proteome analyses under various cellular and physiological conditions. To this end, we here describe methods to isolate centrosomes from human cells and strategies to selectively identify and study the properties of the associated proteins using quantitative mass spectrometry-based proteomics.

Place, publisher, year, edition, pages
Academic Press, 2013. 371-393 p.
, Methods in Enzymology, ISSN 0076-6879 ; 525
Keyword [en]
Absolute Quantification, Primary Cilia, Protein, Digestion, Centriole, Disease, Identification, Duplication, Biogenesis, Components
National Category
Biochemistry and Molecular Biology
URN: urn:nbn:se:kth:diva-123448DOI: 10.1016/B978-0-12-397944-5.00018-3ISI: 000318737400019ScopusID: 2-s2.0-84875443201ISBN: 978-0-12-397944-5OAI: diva2:630007

QC 20130618

Available from: 2013-06-18 Created: 2013-06-10 Last updated: 2013-06-18Bibliographically approved

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