Design of an In-vitro Set-up for Sonothrombolysis of human blood clots using microbubbles
Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesis
Several studies suggest that the use of ultrasound in conjunction with microbubbles (MBs) can induce the lysis of the blood clots through acoustic cavitation and through the production of microjets and microstreaming. However, there is no accordance about the optimal ultrasound parameters that have to be considered in order to achieve the maximum thrombolytic effect, neither a clear agreement about the type of MBs that have to be used.
This project had two main goals: the design and optimization of an in-vitro set-up for the study of clot lysis within coronary arteries and its testing with ultrasound in conjunction with two different types of MBs. The MBs considered were the 3MiCRON MBs and the SonoVue MBs.
The ultrasound sequence was developed using a programmable ultrasound architecture (Verasonics, Inc) and was tested using commercially available clinical transducers.
Using the designed set-up and varying the ultrasound parameters (frequency, pulse length and pulse amplitude) it was possible to study the clot lysis effciency in conjunction with the two types of MBs. For the 3MiCRON MBs no increase in clot lysis was found with the implemented ultrasound parameters, while considering the SonoVue MBs, a 10% increase in clot lysis was found with 10ms long pulse delivered at 50V (peak-to peak value).
The obtained set-up had several aspects in common with the real situation of occluded coronary arteries, although some limitations were present and further optimizations are required.
Further work is required in order to assess if different combination of ultrasound parameters are able to lead to an increase in clot lysis when delivered with 3MiCRON or SonoVue MBs.
Place, publisher, year, edition, pages
2013. , 99 p.
Ultrasound, microbubbles, thrombolysis
IdentifiersURN: urn:nbn:se:kth:diva-123781OAI: oai:DiVA.org:kth-123781DiVA: diva2:630125
Subject / course
Master of Science - Medical Engineering
Bjällmark, AnnaLarsson, Malin
Colarieti-Tosti, Massimiliano, PhD