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Hyaluronic acid-based hydrogel enhances neuronal survival in spinal cord slice cultures from postnatal mice
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2013 (English)In: Journal of biomaterials applications, ISSN 0885-3282, E-ISSN 1530-8022, Vol. 28, no 6, 825-836 p.Article in journal (Refereed) Published
Abstract [en]

Numerous biomaterials based on extracellular matrix-components have been developed. It was our aim to investigate whether a hyaluronic acid-based hydrogel improves neuronal survival and tissue preservation in organotypic spinal cord slice cultures. Organotypic spinal cord slice cultures were cultured for 4 days in vitro (div), either on hyaluronic acid-based hydrogel (hyaluronic acid-gel group), collagen gel (collagen group), directly on polyethylene terephthalate membrane inserts (control group), or in the presence of soluble hyaluronic acid (soluble hyaluronic acid group). Cultures were immunohistochemically stained against neuronal antigen NeuN and analyzed by confocal laser scanning microscopy. Histochemistry for choline acetyltransferance, glial fibrillary acidic protein, and Griffonia simplicifolia isolectin B4 followed by quantitative analysis was performed to assess motorneurons and different glial populations. Confocal microscopic analysis showed a 4-fold increase in the number of NeuN-positive neurons in the hyaluronic acid-gel group compared to both collagen (p < 0.001) and control groups (p < 0.001). Compared to controls, organotypic spinal cord slice cultures maintained on hyaluronic acid-based hydrogel showed 5.9-fold increased survival of choline acetyltransferance-positive motorneurons (p = 0.008), 2-fold more numerous resting microglial cells in the white matter (p = 0.031), and a 61.4% reduction in the number of activated microglial cells within the grey matter (p = 0.05). Hyaluronic acid-based hydrogel had a shear modulus (Gā€²) of ā‰ˆ1200 Pascals (Pa), which was considerably higher than the ā‰ˆ25 Pa measured for collagen gel. Soluble hyaluronic acid failed to improve tissue preservation. In conclusion, hyaluronic acid-based hydrogel improves neuronal and - most notably - motorneuron survival in organotypic spinal cord slice cultures and microglial activation is limited. The positive effects of hyaluronic acid-based hydrogel may at least in part be due to its mechanical properties.

Place, publisher, year, edition, pages
2013. Vol. 28, no 6, 825-836 p.
Keyword [en]
apoptosis, extracellular matrix-based biomaterials, hyaluronic acid-based hydrogel, microglial cells, motorneurons, Neuroprotection
National Category
Biomaterials Science
Identifiers
URN: urn:nbn:se:kth:diva-124760DOI: 10.1177/0885328213483636ISI: 000329317100003Scopus ID: 2-s2.0-84891606942OAI: oai:DiVA.org:kth-124760DiVA: diva2:638408
Note

QC 20140320

Available from: 2013-07-30 Created: 2013-07-30 Last updated: 2017-12-06Bibliographically approved

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Rojas, Ramiro

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