Chemical fragmentation for massively parallel sequencing library preparation
2013 (English)In: Journal of Biotechnology, ISSN 0168-1656, E-ISSN 1873-4863, Vol. 168, no 1, 95-100 p.Article in journal (Refereed) Published
Fragmentation is essential in most library preparation protocols for use with massively parallel sequencing systems. Complexes that generate hydroxyl radicals, such as iron-EDTA, can be used to introduce random DNA cleavage. Here we describe a chemical fragmentation method that can be incorporated into library preparation protocols for next-generation sequencing workflows. This protocol has been validated by whole genome, amplicon and exome sequencing. Chemical fragmentation is a cost-effective alternative to current fragmentation methods that has no observable sequence bias and requires no instrumentation.
Place, publisher, year, edition, pages
2013. Vol. 168, no 1, 95-100 p.
Fragmentation, High-throughput sequencing, Library preparation
IdentifiersURN: urn:nbn:se:kth:diva-133269DOI: 10.1016/j.jbiotec.2013.08.020ISI: 000325464300016ScopusID: 2-s2.0-84883827621OAI: oai:DiVA.org:kth-133269DiVA: diva2:660485
FunderScience for Life Laboratory - a national resource center for high-throughput molecular bioscienceSwedish Research CouncilSwedish Foundation for Strategic Research
QC 201310302013-10-302013-10-292014-03-18Bibliographically approved