Hot-water extracts from the inner bark of Norway spruce with immunomodulating activities
2014 (English)In: Carbohydrate Polymers, ISSN 0144-8617, E-ISSN 1879-1344, Vol. 101, no 1, 699-704 p.Article in journal (Refereed) Published
The inner bark of Norway spruce (Picea abies) was sequentially extracted with hot water at 100 degrees C, 140 C and 160 degrees C. The hot-water extracts (IB 100 degrees C, IB 140 degrees C and IB 160 degrees C) contained pectic polysaccharides and showed immunostimulating activities. Structural analyses of their carbohydrate content, including glycosidic linkage analyses, revealed the presence of pectins with a large rhamnogalacturonan RG-I domain ramified with highly-branched arabinans. IB 100 degrees C also contained a large amount of terminal glucosyl residues, indicating the presence of highly substituted polymers. IB 160 degrees C was mainly composed of starch. The hot-water extracts were tested for two biological activities, namely complement fixation and macrophage stimulation. IB 100 degrees C exhibited the highest complement fixation activity, with a 1.7-times higher IC(H)50 than the control pectin, while IB 140 degrees C and IB 160 degrees C gave similar IC(H)50 values as the control. Macrophages were stimulated by IB 100 degrees C and IB 140 degrees C in a dose-dependent manner, but not by IB 160 degrees C. IB 100 degrees C presented the highest activity toward macrophages, comparable to the control pectin.
Place, publisher, year, edition, pages
2014. Vol. 101, no 1, 699-704 p.
Bark, Biorefinery, Immunomodulating activities, Compositional analysis, Pectins
Materials Chemistry Immunology Wood Science
IdentifiersURN: urn:nbn:se:kth:diva-133433DOI: 10.1016/j.carbpol.2013.09.067ISI: 000330494800087ScopusID: 2-s2.0-84886193809OAI: oai:DiVA.org:kth-133433DiVA: diva2:661135
QC 201311042013-10-312013-10-312014-02-27Bibliographically approved