Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
WNT3A promotes hematopoietic or mesenchymal differentiation from hESCs depending on the time of exposure
KTH, School of Biotechnology (BIO), Industrial Biotechnology. Monash Immunology and Stem Cell Laboratories, Monash University, Wellington Road, Clayton, VIC 3800, Australia .
Show others and affiliations
2013 (English)In: Stem Cell Reports, ISSN 2213-6711, Vol. 1, no 1, 53-65 p.Article in journal (Refereed) Published
Abstract [en]

We investigated the role of canonical WNT signaling in mesoderm and hematopoietic development from human embryonic stem cells (hESCs) using a recombinant human protein-based differentiation medium (APEL). In contrast to prior studies using less defined culture conditions, we found that WNT3A alone was a poor inducer of mesoderm. However, WNT3A synergized with BMP4 to accelerate mesoderm formation, increase embryoid body size, and increase the number of hematopoietic blast colonies. Interestingly, inclusion of WNT3A or a GSK3 inhibitor in methylcellulose colony-forming assays at 4 days of differentiation abrogated blast colony formation but supported the generation of mesospheres that expressed genes associated with mesenchymal lineages. Mesospheres differentiated into cells with characteristics of bone, fat, and smooth muscle. These studies identify distinct effects for WNT3A, supporting the formation of hematopoietic or mesenchymal lineages from human embryonic stem cells, depending upon differentiation stage at the time of exposure.

Place, publisher, year, edition, pages
2013. Vol. 1, no 1, 53-65 p.
Keyword [en]
bone morphogenetic protein 4, fat, Flt3 ligand, interleukin 3, interleukin 6, thrombopoietin, vasculotropin, Wnt3a protein
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:kth:diva-133867DOI: 10.1016/j.stemcr.2013.04.002ISI: 000336632000008Scopus ID: 2-s2.0-84881397186OAI: oai:DiVA.org:kth-133867DiVA: diva2:664142
Note

QC 20131114

Available from: 2013-11-14 Created: 2013-11-11 Last updated: 2013-11-14Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textScopus

Search in DiVA

By author/editor
Gertow, Karin
By organisation
Industrial Biotechnology
Biological Sciences

Search outside of DiVA

GoogleGoogle Scholar

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 42 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf