Contribution of antibody-based protein profiling to the human chromosome-centric proteome project (C-HPP)
2013 (English)In: Journal of Proteome Research, ISSN 1535-3893, E-ISSN 1535-3907, Vol. 12, no 6, 2439-2448 p.Article in journal (Refereed) Published
A gene-centric Human Proteome Project has been proposed to characterize the human protein-coding genes in a chromosome-centered manner to understand human biology and disease. Here, we report on the protein evidence for all genes predicted from the genome sequence based on manual annotation from literature (UniProt), antibody-based profiling in cells, tissues and organs and analysis of the transcript profiles using next generation sequencing in human cell lines of different origins. We estimate that there is good evidence for protein existence for 69% (n = 13985) of the human protein-coding genes, while 23% have only evidence on the RNA level and 7% still lack experimental evidence. Analysis of the expression patterns shows few tissue-specific proteins and approximately half of the genes expressed in all the analyzed cells. The status for each gene with regards to protein evidence is visualized in a chromosome-centric manner as part of a new version of the Human Protein Atlas (www.proteinatlas.org).
Place, publisher, year, edition, pages
2013. Vol. 12, no 6, 2439-2448 p.
Antibody-based protein profiling, C-HPP
Biochemistry and Molecular Biology
IdentifiersURN: urn:nbn:se:kth:diva-134163DOI: 10.1021/pr300924jISI: 000320298600010PubMedID: 23276153ScopusID: 2-s2.0-84879327718OAI: oai:DiVA.org:kth-134163DiVA: diva2:665567
FunderKnut and Alice Wallenberg FoundationEU, FP7, Seventh Framework ProgrammeScience for Life Laboratory - a national resource center for high-throughput molecular bioscience
QC 201311202013-11-202013-11-182016-03-03Bibliographically approved