On-chip acoustic sample preparation for cell studies and diagnostics
2013 (English)In: Proceedings of Meetings on Acoustics: Volume 19, 2013, Acoustical Society of America (ASA), 2013, 1-3 p.Conference paper (Refereed)
We describe a novel platform for acoustic sample preparation in microchannels and microplates. The utilized method is based on generating a multitude of acoustic resonances at a set of different frequencies in microstructures, in order to accurately control the migration and positioning of particles and cells suspended in fluid channels and chambers. The actuation frequencies range from 30 kHz to 7 MHz, which are applied simultaneously and/or in sweeps. We present two devices: A closed microfluidic chip designed for pre-alignment, size-based separation, isolation, up-concentration and lysis of cells, and an open multi-well microplate designed for parallel aggregation and positioning of cells. Both devices in the platform are compatible with high-resolution live-cell microscopy, which is used for fluorescence-based optical characterization. Two bioapplications are demonstrated for each of the devices: The first device is used for size-selective cell isolation and lysis for DNA-based diagnostics, and the second device is used for quantifying the heterogeneity in cytotoxic response of natural killer cells interacting with cancer cells.
Place, publisher, year, edition, pages
Acoustical Society of America (ASA), 2013. 1-3 p.
, Proceedings of Meetings on Acoustics, ISSN 1939-800X ; 19
Acoustic resonance, Bioapplications, Cytotoxic response, Different frequency, Microfluidic chip, Natural killer cells, Optical characterization, Sample preparation
IdentifiersURN: urn:nbn:se:kth:diva-134244DOI: 10.1121/1.4800637ScopusID: 2-s2.0-84878982614OAI: oai:DiVA.org:kth-134244DiVA: diva2:665898
21st International Congress on Acoustics, ICA 2013 - 165th Meeting of the Acoustical Society of America; Montreal, QC; Canada; 2 June 2013 through 7 June 2013
QC 201311212013-11-212013-11-202013-11-21Bibliographically approved