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Identification and verification of microRNAs by high-throughput sequencing
KTH, School of Biotechnology (BIO), Gene Technology.
KTH, School of Biotechnology (BIO), Gene Technology.
KTH, School of Biotechnology (BIO), Gene Technology.
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2013 (English)In: Dictyostelium discoideum Protocols / [ed] Ludwig Eichinger, Francisco Rivero, New York: Humana Press, 2013, 2, 125-138 p.Chapter in book (Refereed)
Abstract [en]

High-throughput sequencing methods have become invaluable for detection and analysis of small RNAs. The results are millions of sequences that need to be carefully analyzed by computational methods and preferentially verified by different experimental techniques. Here we describe how to use high-throughput sequencing followed by bioinformatics and northern blot to identify one particular class of small RNA, microRNAs.

Place, publisher, year, edition, pages
New York: Humana Press, 2013, 2. 125-138 p.
, Methods in Molecular Biology, ISSN 1064-3745 ; 983
Keyword [en]
Bioinformatics, High-throughput sequencing, MicroRNA, Northern blot, Small RNA, RNA induced silencing complex, article, binding affinity, cell protection, chromosome, Dictyostelium discoideum, gene expression, gene interaction, high throughput sequencing, human, priority journal, RNA degradation, RNA translation, Animals, Blotting, Northern, Computational Biology, Dictyostelium, Electrophoresis, Polyacrylamide Gel, High-Throughput Nucleotide Sequencing, MicroRNAs, RNA, Protozoan, Sequence Analysis, RNA
National Category
Biological Sciences
URN: urn:nbn:se:kth:diva-134476DOI: 10.1007/978-1-62703-302-2-7ScopusID: 2-s2.0-84877139952ISBN: 9781627033015OAI: diva2:668103

QC 20131128

Available from: 2013-11-28 Created: 2013-11-25 Last updated: 2013-11-28Bibliographically approved

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Hällman, JimmieReimegård, JohanKäller, Max
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