Identification and verification of microRNAs by high-throughput sequencing
2013 (English)In: Dictyostelium discoideum Protocols / [ed] Ludwig Eichinger, Francisco Rivero, New York: Humana Press, 2013, 2, 125-138 p.Chapter in book (Refereed)
High-throughput sequencing methods have become invaluable for detection and analysis of small RNAs. The results are millions of sequences that need to be carefully analyzed by computational methods and preferentially verified by different experimental techniques. Here we describe how to use high-throughput sequencing followed by bioinformatics and northern blot to identify one particular class of small RNA, microRNAs.
Place, publisher, year, edition, pages
New York: Humana Press, 2013, 2. 125-138 p.
, Methods in Molecular Biology, ISSN 1064-3745 ; 983
Bioinformatics, High-throughput sequencing, MicroRNA, Northern blot, Small RNA, RNA induced silencing complex, article, binding affinity, cell protection, chromosome, Dictyostelium discoideum, gene expression, gene interaction, high throughput sequencing, human, priority journal, RNA degradation, RNA translation, Animals, Blotting, Northern, Computational Biology, Dictyostelium, Electrophoresis, Polyacrylamide Gel, High-Throughput Nucleotide Sequencing, MicroRNAs, RNA, Protozoan, Sequence Analysis, RNA
IdentifiersURN: urn:nbn:se:kth:diva-134476DOI: 10.1007/978-1-62703-302-2-7ScopusID: 2-s2.0-84877139952ISBN: 9781627033015OAI: oai:DiVA.org:kth-134476DiVA: diva2:668103
QC 201311282013-11-282013-11-252013-11-28Bibliographically approved