Revealing Spectral Features in Two-Photon Absorption Spectrum of Hoechst 33342: A Combined Experimental and Quantum-Chemical Study
2013 (English)In: Journal of Physical Chemistry B, ISSN 1520-6106, E-ISSN 1520-5207, Vol. 117, no 40, 12013-12019 p.Article in journal (Refereed) Published
We present the results of wide spectral range Z-scan measurements of the two-photon absorption (2PA) spectrum of the Hoechst 33342 dye. The strongest 2PA of the dye in aqueous solution is found at 575 nm, and the associated two-photon absorption cross section is 245 GM. A weak but clearly visible 2PA band at similar to 850 nm is also observed, a feature that could not be anticipated from the one-photon absorption spectrum. On the basis of the results of hybrid quantum mechanics/molecular mechanics calculations, we put forward a notion that the long-wavelength feature observed in the two-photon absorption spectrum of Hoechst 33342 is due to the formation of dye aggregates.
Place, publisher, year, edition, pages
2013. Vol. 117, no 40, 12013-12019 p.
Optical Nonlinearities, DNA, Binding, Microscopy, Excitation
IdentifiersURN: urn:nbn:se:kth:diva-134741DOI: 10.1021/jp407144kISI: 000326367000012ScopusID: 2-s2.0-84885631119OAI: oai:DiVA.org:kth-134741DiVA: diva2:669183
QC 201312032013-12-032013-11-282013-12-03Bibliographically approved