Highly multiplexed antibody suspension bead arrays for plasma protein profiling
2013 (English)In: Methods in Molecular Biology, ISSN 1064-3745, Vol. 1023, 137-145 p.Article in journal (Refereed) Published
Alongside the increasing availability of affinity reagents, antibody microarrays have become a powerful tool to screen for target proteins in complex samples. Applying directly labeled samples onto arrays instead of using sandwich assays offers an approach to facilitate a systematic, high-throughput, and flexible exploration of protein profiles in body fluids such as serum or plasma. As an alternative to planar arrays, a system based on color-coded beads for the creation of antibody arrays in suspension has become available to offer a microtiter plate-based option for screening larger number of samples with variable sets of capture reagents. A procedure was established for analyzing biotinylated samples without the necessity to remove excess labeling substance. We have shown that this assay system allows detecting proteins down into lower pico-molar and higher pg/ml levels with dynamic ranges over three orders of magnitude. Presently, this workflow enables the profiling of 384 samples for up to 384 proteins per assay.
Place, publisher, year, edition, pages
2013. Vol. 1023, 137-145 p.
Antibody array, Labeling, Plasma, Serum, Suspension bead array, antibody, plasma protein, antibody blood level, antibody detection, article, centrifugation, fluorescence, heat treatment, microtiter plate assay, priority journal, protein determination, protein immobilization, protein microarray, suspension, workflow
Microbiology in the medical area
IdentifiersURN: urn:nbn:se:kth:diva-133797DOI: 10.1007/978-1-4614-7209-4-8ScopusID: 2-s2.0-84883168792ISBN: 9781461471677OAI: oai:DiVA.org:kth-133797DiVA: diva2:677062
QC 201312092013-12-092013-11-112014-02-21Bibliographically approved