Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
The Stockholm laboratory cryo x-ray microscope: towards cell-cell interaction studies
KTH, School of Engineering Sciences (SCI), Applied Physics, Biomedical and X-ray Physics.
KTH, School of Engineering Sciences (SCI), Applied Physics, Biomedical and X-ray Physics.ORCID iD: 0000-0001-8604-735X
KTH, School of Engineering Sciences (SCI), Applied Physics, Biomedical and X-ray Physics.
KTH, School of Engineering Sciences (SCI), Applied Physics, Biomedical and X-ray Physics.
Show others and affiliations
2013 (English)In: 11th International Conference On X-Ray Microscopy (XRM2012), Institute of Physics (IOP), 2013, 012054- p.Conference paper, Published paper (Refereed)
Abstract [en]

We describe recent improvements in the Stockholm laboratory x-ray microscope and the first experiments aiming towards studies of cell-cell interaction. The shorter exposure time due to a higher brightness laser-plasma source will become of large importance for tomography while the reproducible cryo preparation of few-cell samples is essential for the interaction studies.

Place, publisher, year, edition, pages
Institute of Physics (IOP), 2013. 012054- p.
Series
Journal of Physics Conference Series, ISSN 1742-6588 ; 463
National Category
Other Physics Topics
Identifiers
URN: urn:nbn:se:kth:diva-139243DOI: 10.1088/1742-6596/463/1/012054ISI: 000327949000054Scopus ID: 2-s2.0-84891288729OAI: oai:DiVA.org:kth-139243DiVA: diva2:686921
Conference
11th International Conference on X-ray Microscopy (XRM), AUG 05-10, 2012, Shanghai, China
Note

QC 20140113

Available from: 2014-01-13 Created: 2014-01-08 Last updated: 2017-05-04Bibliographically approved
In thesis
1. Laboratory Soft X-Ray Cryo Microscopy: Source, System and Bio Applications
Open this publication in new window or tab >>Laboratory Soft X-Ray Cryo Microscopy: Source, System and Bio Applications
2017 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Soft x-ray microscopes routinely perform high-resolution 3D imaging of biological cells in their near-native environment with short exposure times at synchrotron radiation facilities. Some laboratory-sized microscopes are aiming to make this imaging technique more accessible to a wider scientific community. However, these systems have been hampered by source instabilities hindering routine imaging of biological samples with short exposure times.

This Thesis presents work performed on the Stockholm laboratory x-ray microscope. A novel heat control system has been implemented, improving the stability of the laser-produced plasma source. In combination with recent upgrades to the imaging system and an improved cryofixation method, the microscope now has the capability to routinely produce images with 10-second exposure time of cryofixed biological samples. This has allowed for tomographic imaging of cell autophagy and cell-cell interactions. Furthermore, a numerical 3D image formation model is presented as well as a novel reconstruction approach dealing with the limited depth of focus in x-ray microscopes.

Place, publisher, year, edition, pages
Stockholm: KTH Royal Institute of Technology, 2017. 63 p.
Series
TRITA-FYS, ISSN 0280-316X ; 2017:21
Keyword
X-ray microscopy, X-ray optics, Instrumentation, Laser plasma, Cryofixation, Tomography, Tomographic reconstruction, Autophagy, Immune synapse, Physics
National Category
Physical Sciences
Research subject
Physics
Identifiers
urn:nbn:se:kth:diva-206428 (URN)978-91-7729-369-9 (ISBN)
Public defence
2017-06-02, FD5, Roslagstullsbacken 21, Stockholm, 13:00 (English)
Opponent
Supervisors
Funder
Swedish Research CouncilKnut and Alice Wallenberg Foundation
Note

QC 20170505

Available from: 2017-05-05 Created: 2017-05-04 Last updated: 2017-05-05Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textScopus

Authority records BETA

Selin, MårtenHertz, Hans M.

Search in DiVA

By author/editor
Fogelqvist, EmelieSelin, MårtenMartz, Dale H.Christakou, Athanasia E.Hertz, Hans M.
By organisation
Biomedical and X-ray Physics
Other Physics Topics

Search outside of DiVA

GoogleGoogle Scholar

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 80 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf