Unveiling the structure and ultrastructure of lignin carbohydrate complexes in softwoods
2013 (English)In: International Journal of Biological Macromolecules, ISSN 0141-8130, E-ISSN 1879-0003, Vol. 62, 705-713 p.Article in journal (Refereed) Published
Lignocellulose-based polymers are presently being investigated for potential as raw materials for polymer industry. However, covalent bonds between carbohydrates and lignin, so called lignin carbohydrate complexes (LCCs) present one of the factors impeding clean fractionation of the polymers. Understanding the chemical structure of LCCs in relation to the cell wall ultra-structure therefore provides relevant insight for technical fractionation. In the present work, new mild analytical protocols for LCCs were developed and fractions thereof subjected to detailed structural/ultra-structural characterization. When combined with size exclusion chromatography, the application of mono-component ploysaccharidases proved to be crucial for the identification of LCC moieties and provided indirect evidence for existence of lignin-carbohydrate bonds. The carbohydrate composition of the LCC moieties was essential to determination of the ultra-structural origin of the LCC types. Analytical 31P NMR of the LCCs and of their thioacidolysis products was useful in unveiling lignin structure in LCCs. A complete structure/ultra-structure relationship of LCCs in softwood was determined.
Place, publisher, year, edition, pages
2013. Vol. 62, 705-713 p.
Cell wall LCCs, Lignin carbohydrate complexes (LCCs), Middle lamella LCCs, Mono-component enzymes, PH neutral fractionation, beta mannosidase, galacturonic acid, glucan synthase, lignocellulose, polygalacturonase, xylan endo 1, 3 beta xylosidase, alkalinity, article, bromination, carbohydrate analysis, carbohydrate metabolism, centrifugation, dissolution, fractionation, gel permeation chromatography, hydrolysis, pH, quantitative analysis, structure analysis, ultrastructure, wood
IdentifiersURN: urn:nbn:se:kth:diva-139988DOI: 10.1016/j.ijbiomac.2013.10.022ISI: 000329881200104ScopusID: 2-s2.0-84887201280OAI: oai:DiVA.org:kth-139988DiVA: diva2:689135
FunderKnut and Alice Wallenberg Foundation
QC 201401202014-01-202014-01-162014-02-17Bibliographically approved