Structure and composition of biofilm communities in a moving bed biofilm reactor for nitritation-anammox at low temperatures
2014 (English)In: Bioresource Technology, ISSN 0960-8524, E-ISSN 1873-2976, Vol. 154, 267-273 p.Article in journal (Refereed) Published
It is a challenge to apply anaerobic ammonium oxidation (anammox) for nitrogen removal from wastewater at low temperatures. Maintenance of anammox- and aerobic ammonia oxidizing bacteria (AOB) and suppression of nitrite oxidizing bacteria (NOB) are key issues. In this work, a nitritation-anammox moving bed biofilm pilot reactor was operated at 19-10°C for 300d. Nitrogen removal was decreasing, but stable, at 19-13°C. At 10°C removal became unstable. Quantitative PCR, fluorescence in situ hybridization and gene sequencing showed that no major microbial community changes were observed with decreased temperature. Anammox bacteria dominated the biofilm (0.9-1.2×1014 16S rRNA copies m-2). Most anammox bacteria were similar to Brocadia sp. 40, but another smaller Brocadia population was present near the biofilm-water interface, where also the AOB community (Nitrosomonas) was concentrated in thin layers (1.8-5.3×1012 amoA copies m-2). NOB (Nitrobacter, Nitrospira) were always present at low concentrations (<1.3×1011 16S rRNA copies m-2).
Place, publisher, year, edition, pages
2014. Vol. 154, 267-273 p.
FISH-CLSM, Low temperature, MBBR, Microbial community analysis, Nitritation-anammox
Other Environmental Engineering
IdentifiersURN: urn:nbn:se:kth:diva-140806DOI: 10.1016/j.biortech.2013.12.062ISI: 000331461600037ScopusID: 2-s2.0-84891820748OAI: oai:DiVA.org:kth-140806DiVA: diva2:693234
FunderFormas, 211-2010-140 243-2010-2259
QC 201402042014-02-042014-01-312014-03-24Bibliographically approved