Development and fabrication of OSTE and OSTE+ cartridges for the integration of quartz crystal microbalance biosensors
Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesis
The introduction of lab-on-a chip (LOC) devices introduced hopes of more advanced and rapid point-of-care diagnostics and pathogen detection, especially when used in combination with miniature and sensitive biosensors. Nevertheless, the commercialization of such devices has been limited, partly due to issues in achieving a facile and economically viable packaging using cost-effective materials. Newly developed thermosetting polymers address some of the issues by offering industry like material properties (i.e. thermoplastics) along with straight-forward back-end processing, e.g. adaptable surface modification and low temperature dry-bonding. Thus, such polymers have a great potential to be used for the fabrication of LOC-devices with integrated biosensors.
In this thesis project, a novel approach for the integration and packaging of quartz crystal microbalance (QCM) biosensor chips, aimed for Norovirus detection, has been developed. The work initially evaluates the properties of novel thermosetting polymers for LOC cartridge applications. The study continues with an investigation of new polymer formulations, which features more favorable material properties and versatile back-end processing compared to standard OSTE. Lastly, microfluidic cartridges based on a novel thermosetting polymer were successfully fabricated and integrated with a gold-coated quartz crystal. The bond interfaces were homogenous, void-free and leak-tight. The devices were successfully tested in terms of noise, drift and response in a flow-through setting and upon exposure of bovine serum albumin protein.
Place, publisher, year, edition, pages
OSTE, OSTE+, biosensor, QCM
Other Electrical Engineering, Electronic Engineering, Information Engineering
IdentifiersURN: urn:nbn:se:kth:diva-141132OAI: oai:DiVA.org:kth-141132DiVA: diva2:695057
Subject / course
Master of Science - Nanotechnology
2013-05-03, Q26, Osquldas väg 4,, Stockholm, 14:15 (English)
Sandström, Niklas, PhD student
van der Wijngaart, Wouter, PROFESSOR