Characterization of VCAM-1-Binding Peptide-Functionalized Quantum Dots for Molecular Imaging of Inflamed Endothelium
2013 (English)In: PLoS ONE, ISSN 1932-6203, Vol. 8, no 12, e83805- p.Article in journal (Refereed) Published
Inflammation-induced activation of endothelium constitutes one of the earliest changes during atherogenesis. New imaging techniques that allow detecting activated endothelial cells can improve the identification of persons at high cardiovascular risk in early stages. Quantum dots (QDs) have attractive optical properties such as bright fluorescence and high photostability, and have been increasingly studied and developed for bio-imaging and bio-targeting applications. We report here the development of vascular cell adhesion molecule-1 binding peptide (VCAM-1 binding peptide) functionalized QDs (VQDs) from amino QDs. It was found that the QD fluorescence signal in tumor necrosis factor alpha (TNF-alpha) treated endothelial cells in vitro was significantly higher when these cells were labeled with VQDs than amino QDs. The VQD labeling of TNF-alpha-treated endothelial cells was VCAM-1 specific since pre-incubation with recombinant VCAM-1 blocked cells' uptake of VQDs. Our ex vivo and in vivo experiments showed that in the inflamed endothelium, QD fluorescence signal from VQDs was also much stronger than that of amino QDs. Moreover, we observed that the QD fluorescence peak was significantly blue-shifted after VQDs interacted with aortic endothelial cells in vivo and in vitro. A similar blue-shift was observed after VQDs were incubated with recombinant VCAM-1 in tube. We anticipate that the specific interaction between VQDs and VCAM-1 and the blue-shift of the QD fluorescence peak can be very useful for VCAM-1 detection in vivo.
Place, publisher, year, edition, pages
2013. Vol. 8, no 12, e83805- p.
IdentifiersURN: urn:nbn:se:kth:diva-141075DOI: 10.1371/journal.pone.0083805ISI: 000329325200127ScopusID: 2-s2.0-84896730935OAI: oai:DiVA.org:kth-141075DiVA: diva2:695328
FunderVinnova, P35914-1Swedish Foundation for Strategic Research Swedish Research Council, 621-2011-4381Science for Life Laboratory - a national resource center for high-throughput molecular bioscience
QC 201402102014-02-102014-02-072015-03-06Bibliographically approved