Microchip screening platform for assessment of cytotoxic effector cells
(English)Manuscript (preprint) (Other academic)
Here we report a screening platform for assessment of the cytotoxic potential of individual natural killer (NK) or T cells within larger populations. Human primary NK cells or human Epstein-Barr virus (EBV)- specific T cells were distributed across a silicon-glass microchip containing 32 400 individual microwells loaded with target cells. Through fluorescence screening and automated image analysis the numbers of effector and live or dead target cells in each well could be assessed at different time-points after initial mixing. Cytotoxicity was also studied by time-lapse live-cell imaging in microwells quantifying the killing potential of individual NK cells. Although most resting NK cells (≈75%) were non-cytotoxic to the leukemia cell line K562, some NK cells were able to kill several (≥3) target cells within the 12 hours long experiment. We demonstrate that this assay can be used to enumerate and characterize cytotoxic cells, something that could find clinical applications, e.g. in the selection of donors for stem cell transplantation or generation of highly specific and cytotoxic cells for adoptive immunotherapy.
Immunology in the medical area
Research subject Biological Physics
IdentifiersURN: urn:nbn:se:kth:diva-142469OAI: oai:DiVA.org:kth-142469DiVA: diva2:703042
FunderSwedish Foundation for Strategic Research Swedish Cancer SocietySwedish Research Council
QS 20142014-03-052014-03-052014-03-06Bibliographically approved