Quantitative Proteomics Reveals that Plasma Membrane Microdomains From Poplar Cell Suspension Cultures Are Enriched in Markers of Signal Transduction, Molecular Transport, and Callose Biosynthesis
2013 (English)In: Molecular & Cellular Proteomics, ISSN 1535-9476, E-ISSN 1535-9484, Vol. 12, no 12, 3874-3885 p.Article in journal (Refereed) Published
The plasma membrane (PM) is a highly dynamic interface that contains detergent-resistant microdomains (DRMs). The aim of this work was to determine the main functions of such microdomains in poplar through a proteomic analysis using gel-based and solution (iTRAQ) approaches. A total of 80 proteins from a limited number of functional classes were found to be significantly enriched in DRM relative to PM. The enriched proteins are markers of signal transduction, molecular transport at the PM, or cell wall biosynthesis. Their intrinsic properties are presented and discussed together with the biological significance of their enrichment in DRM. Of particular importance is the significant and specific enrichment of several callose [(1→3)-β-glucan] synthase isoforms, whose catalytic activity represents a final response to stress, leading to the deposition of callose plugs at the surface of the PM. An integrated functional model that connects all DRM-enriched proteins identified is proposed. This report is the only quantitative analysis available to date of the protein composition of membrane microdomains from a tree species.
Place, publisher, year, edition, pages
2013. Vol. 12, no 12, 3874-3885 p.
Detergent-Resistant Membranes, Arabidopsis-Thaliana, Lipid Rafts, Statistical-Model, Phospholipase-D, Plant Biology, Proteins, Identification, Synthase, Tobacco
Biochemistry and Molecular Biology
IdentifiersURN: urn:nbn:se:kth:diva-142525DOI: 10.1074/mcp.M113.029033ISI: 000329993600034ScopusID: 2-s2.0-84890713894OAI: oai:DiVA.org:kth-142525DiVA: diva2:703336
FunderFormasSwedish Foundation for Strategic Research
QC 201403062014-03-062014-03-062014-07-10Bibliographically approved