Magnetic bead assisted labeling of antibodies at nanogram scale
2014 (English)In: Proteomics, ISSN 1615-9853, E-ISSN 1615-9861, Vol. 14, no 1, 14-18 p.Article in journal (Refereed) Published
There are currently several initiatives that aim to produce binding reagents for proteome-wide analysis. To enable protein detection, visualization, and target quantification, covalent coupling of reporter molecules to antibodies is essential. However, current labeling protocols recommend considerable amount of antibodies, require antibody purity and are not designed for automation. Given that small amounts of antibodies are often sufficient for downstream analysis, we developed a labeling protocol that combines purification and modification of antibodies at submicrogram quantities. With the support of magnetic microspheres, automated labeling of antibodies in parallel using biotin or fluorescent dyes was achieved.
Place, publisher, year, edition, pages
2014. Vol. 14, no 1, 14-18 p.
Antibody, Automated protocol, Biotinylation, Labeling, Nanogram scale, Technology
Biochemistry and Molecular Biology
IdentifiersURN: urn:nbn:se:kth:diva-142519DOI: 10.1002/pmic.201300283ISI: 000330727000003ScopusID: 2-s2.0-84892410462OAI: oai:DiVA.org:kth-142519DiVA: diva2:703400
FunderVinnovaKnut and Alice Wallenberg FoundationScience for Life Laboratory - a national resource center for high-throughput molecular bioscience
QC 201403062014-03-062014-03-062015-02-03Bibliographically approved