Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
CE analysis of single wood cells performing hydrolysis and preconcentration in open microchannels
KTH, School of Chemical Science and Engineering (CHE), Chemistry, Applied Physical Chemistry.
KTH, School of Chemical Science and Engineering (CHE), Chemistry, Applied Physical Chemistry.
KTH, School of Chemical Science and Engineering (CHE), Chemistry, Applied Physical Chemistry.ORCID iD: 0000-0003-3548-217X
Show others and affiliations
2014 (English)In: Electrophoresis, ISSN 0173-0835, E-ISSN 1522-2683, Vol. 35, no 2-3, 450-457 p.Article in journal (Refereed) Published
Abstract [en]

In the present work, monosaccharides from pulp samples and single wood fibers were analyzed with CE, using indirect detection due to the lack of chromophores on the monosaccharides. The hydrolysis degradation of cellulose and hemicellulose into monosaccharides was performed using TFA, either in bulk scale or in microscale. In the microscale, one single wood fiber was hydrolyzed in an open microchannel manufactured on a silicon microchip with the dimensions 50 μm × 50 μm (length 1 or 3 cm). The low monosaccharide amounts derived from a single fiber implied that a preconcentration step was necessary to increase the detectability. Thus, an electromigration preconcentration of the hydrolyzed samples was performed within the microchannel, which resulted in a significantly enhanced signal intensity of the monosaccharides. In addition to the experimental study, computer simulations were performed regarding the preconcentration step of monosaccharides. The results from these simulations correlated well with the experimental results.

Place, publisher, year, edition, pages
2014. Vol. 35, no 2-3, 450-457 p.
Keyword [en]
Capillary electrophoresis, Open microchannels, Preconcentration, Wood cells
National Category
Other Chemistry Topics Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:kth:diva-143085DOI: 10.1002/elps.201300408ISI: 000331899400028Scopus ID: 2-s2.0-84892478458OAI: oai:DiVA.org:kth-143085DiVA: diva2:705440
Funder
Swedish Research Council, 621-2009-4095
Note

QC 20140317

Available from: 2014-03-17 Created: 2014-03-17 Last updated: 2017-12-05Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textScopus

Authority records BETA

Jacksén, JohanEmmer, Åsa

Search in DiVA

By author/editor
Rokhas, Maria KhihonMikkonen, SaaraJacksén, JohanEmmer, Åsa
By organisation
Applied Physical Chemistry
In the same journal
Electrophoresis
Other Chemistry TopicsBiochemistry and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 93 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf