CE analysis of single wood cells performing hydrolysis and preconcentration in open microchannels
2014 (English)In: Electrophoresis, ISSN 0173-0835, E-ISSN 1522-2683, Vol. 35, no 2-3, 450-457 p.Article in journal (Refereed) Published
In the present work, monosaccharides from pulp samples and single wood fibers were analyzed with CE, using indirect detection due to the lack of chromophores on the monosaccharides. The hydrolysis degradation of cellulose and hemicellulose into monosaccharides was performed using TFA, either in bulk scale or in microscale. In the microscale, one single wood fiber was hydrolyzed in an open microchannel manufactured on a silicon microchip with the dimensions 50 μm × 50 μm (length 1 or 3 cm). The low monosaccharide amounts derived from a single fiber implied that a preconcentration step was necessary to increase the detectability. Thus, an electromigration preconcentration of the hydrolyzed samples was performed within the microchannel, which resulted in a significantly enhanced signal intensity of the monosaccharides. In addition to the experimental study, computer simulations were performed regarding the preconcentration step of monosaccharides. The results from these simulations correlated well with the experimental results.
Place, publisher, year, edition, pages
2014. Vol. 35, no 2-3, 450-457 p.
Capillary electrophoresis, Open microchannels, Preconcentration, Wood cells
Other Chemistry Topics Biochemistry and Molecular Biology
IdentifiersURN: urn:nbn:se:kth:diva-143085DOI: 10.1002/elps.201300408ISI: 000331899400028ScopusID: 2-s2.0-84892478458OAI: oai:DiVA.org:kth-143085DiVA: diva2:705440
FunderSwedish Research Council, 621-2009-4095
QC 201403172014-03-172014-03-172014-04-07Bibliographically approved