Real-time study of shape and thermal fluctuations in the echinocyte transformation of human erythrocytes using defocusing microscopy
2012 (English)In: Journal of Biomedical Optics, ISSN 1083-3668, E-ISSN 1560-2281, Vol. 17, no 10, 106013- p.Article in journal (Refereed) Published
We present a real-time method to measure the amplitude of thermal fluctuations in biological membranes by means of a new treatment of the defocusing microscopy (DM) optical technique. This approach was also applied to study the deformation of human erythrocytes to its echinocyte structure. This was carried out by making three-dimensional shape reconstructions of the cell and measuring the thermal fluctuations of its membrane, as the cell is exposed to the anti-inflammatory drug naproxen and as it recovers its original shape, when it is subsequently cleansed of the drug. The results showed biomechanical changes in the membrane even at low naproxen concentration (0.2 mM). Also, we found that when the cell recovered its original shape, the membrane properties were different compared to the nondrugged initial erythrocyte, indicating that the drug administration-recovery process is not completely reversible.
Place, publisher, year, edition, pages
2012. Vol. 17, no 10, 106013- p.
red blood cells, erythrocyte, membrane dynamics, bright field microscopy
Biochemistry and Molecular Biology
Research subject Biological Physics
IdentifiersURN: urn:nbn:se:kth:diva-143348DOI: 10.1117/1.JBO.17.10.106013ISI: 000309910700040ScopusID: 2-s2.0-84876557872OAI: oai:DiVA.org:kth-143348DiVA: diva2:706315
QC 201409192014-03-192014-03-192014-09-19Bibliographically approved