Biodegradable polymeric vesicles containing magnetic nanoparticles, quantum dots and anticancer drugs for drug delivery and imaging
2014 (English)In: Biomaterials, ISSN 0142-9612, E-ISSN 1878-5905, Vol. 35, no 12, 3885-3894 p.Article in journal (Refereed) Published
We have developed biodegradable polymeric vesicles as a nanocarrier system for multimodal bioimaging and anticancer drug delivery. The poly(lactic-co-glycolic acid) (PLGA) vesicles were fabricated by encapsulating inorganic imaging agents of superparamagnetic iron oxide nanoparticles (SPION), manganese-doped zinc sulfide (Mn:ZnS) quantum dots (QDs) and the anticancer drug busulfan into PLGA nanoparticles via an emulsion-evaporation method. T-2(*)-weighted magnetic resonance imaging (MRI) of PLGA-SPION-Mn:ZnS phantoms exhibited enhanced negative contrast with r(2)(*) relaxivity of approximately 523 s(-1) mM(-1) Fe. Murine macrophage (J774A) cellular uptake of PLGA vesicles started fluorescence imaging at 2 h and reached maximum intensity at 24 h incubation. The drug delivery ability of PLGA vesicles was demonstrated in vitro by release of busulfan. PLGA vesicle degradation was studied in vitro, showing that approximately 32% was degraded into lactic and glycolic acid over a period of 5 weeks. The biodistribution of PLGA vesicles was investigated in vivo by MRI in a rat model. Change of contrast in the liver could be visualized by MRI after 7 min and maximal signal loss detected after 4 h post-injection of PLGA vesicles. Histological studies showed that the presence of PLGA vesicles in organs was shifted from the lungs to the liver and spleen over time.
Place, publisher, year, edition, pages
2014. Vol. 35, no 12, 3885-3894 p.
Biodegradable polymer, Multifunctional nanoparticles, Anticancer drug delivery, Busulfan, Fluorescence imaging, Magnetic resonance imaging
IdentifiersURN: urn:nbn:se:kth:diva-144352DOI: 10.1016/j.biomaterials.2014.01.041ISI: 000332431700017ScopusID: 2-s2.0-84893816633OAI: oai:DiVA.org:kth-144352DiVA: diva2:713442
FunderSwedish Cancer Society
QC 201404232014-04-232014-04-222016-05-16Bibliographically approved