RNA- and Antibody-Based Profiling of the Human Proteome with Focus on Chromosome 19
2014 (English)In: Journal of Proteome Research, ISSN 1535-3893, E-ISSN 1535-3907, Vol. 13, no 4, 2019-2027 p.Article in journal (Refereed) Published
An important part of the Human Proteome Project is to characterize the protein complement of the genome with antibody-based profiling. Within the framework of this effort, a new version 12 of the Human Protein Atlas (www.proteinatlas.org) has been launched, including transcriptomics data for 27 tissues and 44 cell lines to complement the protein expression data from antibody-based profiling. Besides the extensive addition of transcriptomics data, the Human Protein Atlas now contains antibody-based protein profiles for 82% of the 20 329 putative protein-coding genes. The comprehensive data resulting from RNA-seq analysis and antibody-based profiling performed within the Human Protein Atlas as well as information from UniProt were used to generate evidence summary scores for each of the 20 329 genes, of which 94% now have experimental evidence at least at transcript level. The evidence scores for all individual genes are displayed with regards to both RNA- and antibody-based protein profiles, including chromosome-centric visualizations. An analysis of the human chromosome 19 shows that similar to 43% of the genes are expressed at the transcript level in all 27 tissues analyzed, suggesting a "house-keeping" function, while 12% of the genes show a more tissue-specific pattern with enriched expression in one of the analyzed tissues only.
Place, publisher, year, edition, pages
2014. Vol. 13, no 4, 2019-2027 p.
antibodies, Human Proteome Project, Human Protein Atlas, immunohistochemistry, immunofluorescence, RNA deep sequencing, tissue profiling, transcriptomics, chromosome 19
Biochemistry and Molecular Biology
IdentifiersURN: urn:nbn:se:kth:diva-145089DOI: 10.1021/pr401156gISI: 000334016400022ScopusID: 2-s2.0-84898766939OAI: oai:DiVA.org:kth-145089DiVA: diva2:716194
FunderKnut and Alice Wallenberg FoundationEU, FP7, Seventh Framework ProgrammeScience for Life Laboratory - a national resource center for high-throughput molecular bioscience
QC 201405082014-05-082014-05-082014-05-08Bibliographically approved