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A lateral flow paper microarray for rapid allergy point of care diagnostics
KTH, School of Biotechnology (BIO), Proteomics and Nanobiotechnology. KTH, Centres, Science for Life Laboratory, SciLifeLab.
KTH, School of Biotechnology (BIO), Proteomics and Nanobiotechnology. KTH, Centres, Science for Life Laboratory, SciLifeLab.
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2014 (English)In: The Analyst, ISSN 0003-2654, E-ISSN 1364-5528, Vol. 139, no 10, 2348-2354 p.Article in journal (Refereed) Published
Abstract [en]

There is a growing need for multiplexed specific IgE tests that can accurately evaluate patient sensitization profiles. However, currently available commercial tests are either single/low-plexed or require sophisticated instrumentation at considerable cost per assay. Here, we present a novel convenient lateral flow microarray-based device that employs a novel dual labelled gold nanoparticle-strategy for rapid and sensitive detection of a panel of 15 specific IgE responses in 35 clinical serum samples. Each gold nanoparticle was conjugated to an optimized ratio of HRP and anti-IgE, allowing significant enzymatic amplification to improve the sensitivity of the assay as compared to commercially available detection reagents. The mean inter-assay variability of the developed LFM assay was 12% CV, and analysis of a cohort of clinical samples (n = 35) revealed good general agreement with ImmunoCAP, yet with a varying performance among allergens (AUC = [0.54-0.88], threshold 1 kU). Due to the rapid and simple procedure, inexpensive materials and read-out by means of a consumer flatbed scanner, the presented assay may provide an interesting low-cost alternative to existing multiplexed methods when thresholds > 1 kU are acceptable.

Place, publisher, year, edition, pages
Royal Society of Chemistry, 2014. Vol. 139, no 10, 2348-2354 p.
Keyword [en]
Gold Nanoparticles, Immunoglobulin-E, Of-Care, Immunoassay, Serum
National Category
Biomedical Laboratory Science/Technology
Identifiers
URN: urn:nbn:se:kth:diva-145615DOI: 10.1039/c3an01806gISI: 000334734200011Scopus ID: 2-s2.0-84898923407OAI: oai:DiVA.org:kth-145615DiVA: diva2:719144
Funder
VinnovaScience for Life Laboratory - a national resource center for high-throughput molecular bioscience
Note

QC 20140523

Available from: 2014-05-23 Created: 2014-05-23 Last updated: 2017-12-05Bibliographically approved

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Chinnasamy, ThiruppathirajaGantelius, JesperAndersson Svahn, Helene
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  • apa
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