A lateral flow paper microarray for rapid allergy point of care diagnostics
2014 (English)In: The Analyst, ISSN 0003-2654, E-ISSN 1364-5528, Vol. 139, no 10, 2348-2354 p.Article in journal (Refereed) Published
There is a growing need for multiplexed specific IgE tests that can accurately evaluate patient sensitization profiles. However, currently available commercial tests are either single/low-plexed or require sophisticated instrumentation at considerable cost per assay. Here, we present a novel convenient lateral flow microarray-based device that employs a novel dual labelled gold nanoparticle-strategy for rapid and sensitive detection of a panel of 15 specific IgE responses in 35 clinical serum samples. Each gold nanoparticle was conjugated to an optimized ratio of HRP and anti-IgE, allowing significant enzymatic amplification to improve the sensitivity of the assay as compared to commercially available detection reagents. The mean inter-assay variability of the developed LFM assay was 12% CV, and analysis of a cohort of clinical samples (n = 35) revealed good general agreement with ImmunoCAP, yet with a varying performance among allergens (AUC = [0.54-0.88], threshold 1 kU). Due to the rapid and simple procedure, inexpensive materials and read-out by means of a consumer flatbed scanner, the presented assay may provide an interesting low-cost alternative to existing multiplexed methods when thresholds > 1 kU are acceptable.
Place, publisher, year, edition, pages
Royal Society of Chemistry, 2014. Vol. 139, no 10, 2348-2354 p.
Gold Nanoparticles, Immunoglobulin-E, Of-Care, Immunoassay, Serum
Biomedical Laboratory Science/Technology
IdentifiersURN: urn:nbn:se:kth:diva-145615DOI: 10.1039/c3an01806gISI: 000334734200011ScopusID: 2-s2.0-84898923407OAI: oai:DiVA.org:kth-145615DiVA: diva2:719144
FunderVinnovaScience for Life Laboratory - a national resource center for high-throughput molecular bioscience
QC 201405232014-05-232014-05-232014-05-23Bibliographically approved