Change search
ReferencesLink to record
Permanent link

Direct link
A lateral flow paper microarray for rapid allergy point of care diagnostics
KTH, School of Biotechnology (BIO), Proteomics and Nanobiotechnology. KTH, Centres, Science for Life Laboratory, SciLifeLab.
KTH, School of Biotechnology (BIO), Proteomics and Nanobiotechnology. KTH, Centres, Science for Life Laboratory, SciLifeLab.
Show others and affiliations
2014 (English)In: The Analyst, ISSN 0003-2654, E-ISSN 1364-5528, Vol. 139, no 10, 2348-2354 p.Article in journal (Refereed) Published
Abstract [en]

There is a growing need for multiplexed specific IgE tests that can accurately evaluate patient sensitization profiles. However, currently available commercial tests are either single/low-plexed or require sophisticated instrumentation at considerable cost per assay. Here, we present a novel convenient lateral flow microarray-based device that employs a novel dual labelled gold nanoparticle-strategy for rapid and sensitive detection of a panel of 15 specific IgE responses in 35 clinical serum samples. Each gold nanoparticle was conjugated to an optimized ratio of HRP and anti-IgE, allowing significant enzymatic amplification to improve the sensitivity of the assay as compared to commercially available detection reagents. The mean inter-assay variability of the developed LFM assay was 12% CV, and analysis of a cohort of clinical samples (n = 35) revealed good general agreement with ImmunoCAP, yet with a varying performance among allergens (AUC = [0.54-0.88], threshold 1 kU). Due to the rapid and simple procedure, inexpensive materials and read-out by means of a consumer flatbed scanner, the presented assay may provide an interesting low-cost alternative to existing multiplexed methods when thresholds > 1 kU are acceptable.

Place, publisher, year, edition, pages
Royal Society of Chemistry, 2014. Vol. 139, no 10, 2348-2354 p.
Keyword [en]
Gold Nanoparticles, Immunoglobulin-E, Of-Care, Immunoassay, Serum
National Category
Biomedical Laboratory Science/Technology
URN: urn:nbn:se:kth:diva-145615DOI: 10.1039/c3an01806gISI: 000334734200011ScopusID: 2-s2.0-84898923407OAI: diva2:719144
VinnovaScience for Life Laboratory - a national resource center for high-throughput molecular bioscience

QC 20140523

Available from: 2014-05-23 Created: 2014-05-23 Last updated: 2014-05-23Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textScopus

Search in DiVA

By author/editor
Chinnasamy, ThiruppathirajaGantelius, JesperAndersson Svahn, Helene
By organisation
Proteomics and NanobiotechnologyScience for Life Laboratory, SciLifeLab
In the same journal
The Analyst
Biomedical Laboratory Science/Technology

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 37 hits
ReferencesLink to record
Permanent link

Direct link