Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Size-dependent cytotoxicity of silver nanoparticles in human lung cells: the role of cellular uptake, agglomeration and Ag release
KTH, School of Chemical Science and Engineering (CHE), Chemistry, Surface and Corrosion Science.ORCID iD: 0000-0002-5263-6487
KTH, School of Chemical Science and Engineering (CHE), Chemistry, Surface and Corrosion Science.ORCID iD: 0000-0003-2206-0082
Show others and affiliations
2014 (English)In: Particle and Fibre Toxicology, ISSN 1743-8977, E-ISSN 1743-8977, Vol. 11, no 1, 11- p.Article in journal (Refereed) Published
Abstract [en]

Background: Silver nanoparticles (AgNPs) are currently one of the most manufactured nanomaterials. A wide range of toxicity studies have been performed on various AgNPs, but these studies report a high variation in toxicity and often lack proper particle characterization. The aim of this study was to investigate size-and coating-dependent toxicity of thoroughly characterized AgNPs following exposure of human lung cells and to explore the mechanisms of toxicity. Methods: BEAS-2B cells were exposed to citrate coated AgNPs of different primary particle sizes (10, 40 and 75 nm) as well as to 10 nm PVP coated and 50 nm uncoated AgNPs. The particle agglomeration in cell medium was investigated by photon cross correlation spectroscopy (PCCS); cell viability by LDH and Alamar Blue assay; ROS induction by DCFH-DA assay; genotoxicity by alkaline comet assay and gamma H(2)AX foci formation; uptake and intracellular localization by transmission electron microscopy (TEM); and cellular dose as well as Ag release by atomic absorption spectroscopy (AAS). Results: The results showed cytotoxicity only of the 10 nm particles independent of surface coating. In contrast, all AgNPs tested caused an increase in overall DNA damage after 24 h assessed by the comet assay, suggesting independent mechanisms for cytotoxicity and DNA damage. However, there was no gamma H(2)AX foci formation and no increased production of intracellular reactive oxygen species (ROS). The reasons for the higher toxicity of the 10 nm particles were explored by investigating particle agglomeration in cell medium, cellular uptake, intracellular localization and Ag release. Despite different agglomeration patterns, there was no evident difference in the uptake or intracellular localization of the citrate and PVP coated AgNPs. However, the 10 nm particles released significantly more Ag compared with all other AgNPs (approx. 24 wt% vs. 4-7 wt%) following 24 h in cell medium. The released fraction in cell medium did not induce any cytotoxicity, thus implying that intracellular Ag release was responsible for the toxicity. Conclusions: This study shows that small AgNPs (10 nm) are cytotoxic for human lung cells and that the toxicity observed is associated with the rate of intracellular Ag release, a 'Trojan horse' effect.

Place, publisher, year, edition, pages
BioMed Central, 2014. Vol. 11, no 1, 11- p.
Keyword [en]
Silver nanoparticles, BEAS-2B cells, Size-dependent toxicity, Cytotoxicity, Genotoxicity, Silver release
National Category
Other Chemistry Topics
Identifiers
URN: urn:nbn:se:kth:diva-145613DOI: 10.1186/1743-8977-11-11ISI: 000334889300001Scopus ID: 2-s2.0-84894377473OAI: oai:DiVA.org:kth-145613DiVA: diva2:719151
Funder
Forte, Swedish Research Council for Health, Working Life and Welfare, 2011-0832Swedish Research Council
Note

QC 20140523

Available from: 2014-05-23 Created: 2014-05-23 Last updated: 2017-12-05Bibliographically approved
In thesis
1. Surface reactivity of metal nanoparticles: - importance of surface active agents and biomolecules from a transformation, mobility and toxicity perspective
Open this publication in new window or tab >>Surface reactivity of metal nanoparticles: - importance of surface active agents and biomolecules from a transformation, mobility and toxicity perspective
2017 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Metallic nanoparticles possess unique properties due to their size and are widely used in e.g. consumer products. From this follows a need to identify and assess potential risks of human and environmental exposure. Their size facilitates uptake in organisms and disruption of various biological processes. Together with a high reactivity, mainly due to their large surface area in solution, they are both commonly used in different applications and of a potential safety concern. Risk assessment requires hence in-depth knowledge on the particle characteristics and their behavior in solution but also how these properties change with time and exposure conditions and whether these characteristics can be linked to toxicity following nanoparticle exposure. This thesis addresses these aspects with a main focus on metal nanoparticles and elaborates on the importance of characterization, how such measurements can be done, and on interactions with surfactants and biomolecules and toxic effects.Silver nanoparticles are, due to their antibacterial properties, often used in sportswear to prevent sweat odor. During laundry they may be dispersed and interact with surfactants of the washing powder, influencing their properties and stability in solution. These aspects are addressed in Papers I, III and V on silver nanoparticles of different size and surface coatings. The stability was shown to depend on the surface charge and the concentration of the surfactant. The stability and extent of silver release were reduced upon sequential exposure, indicating the importance of the particle history on their bioaccessibility, mobility and potential toxicity. A mechanism was proposed for how silver nanoparticles are stabilized in surfactant solutions.Toxic effects of silver nanoparticles of different size and coatings on cultivated lung cells, Paper II, and effects of copper-containing nanoparticles on different blood cells, Paper IV, were studied in vitro. The smallest particles were most cytotoxic and the “Trojan horse” mechanism played an important role, meaning that the nanoparticles facilitate cellular uptake followed by ion-release.Difficulties in the determination and interpretation of the zeta potential, related to the surface charge, of metal nanoparticles in complex solutions are elucidated in Paper VI. Guidelines are provided on how to accurately assess this property.

Place, publisher, year, edition, pages
Stockholm: KTH Royal Institute of Technology, 2017. 62 p.
Series
TRITA-CHE-Report, ISSN 1654-1081 ; 2017:16
Keyword
metal nanoparticles, surfactants, metal release, characterization, size distribution, nanotoxicology, risk assessment
National Category
Chemical Sciences
Research subject
Chemistry
Identifiers
urn:nbn:se:kth:diva-199596 (URN)978-91-7729-247-0 (ISBN)
Public defence
2017-02-02, F3, Lindstedtsvägen 26, Stockholm, 10:00 (English)
Opponent
Supervisors
Funder
Swedish Research CouncilSwedish Research Council Formas
Note

QC 20170111

Available from: 2017-01-11 Created: 2017-01-10 Last updated: 2017-03-07Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textScopus

Authority records BETA

Odnevall Wallinder, Inger

Search in DiVA

By author/editor
Skoglund, SaraOdnevall Wallinder, Inger
By organisation
Surface and Corrosion Science
In the same journal
Particle and Fibre Toxicology
Other Chemistry Topics

Search outside of DiVA

GoogleGoogle Scholar

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 99 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf