Nanoscale analysis of cell-matrix adhesions
(English)Manuscript (preprint) (Other academic)
Cell adhesion to the extracellular matrix is required for physiological processes, such as morphogenesis and wound healing. The cell adheres to the extracellular matrix via focal adhesions, which are considered to be cell adhesion organelles that govern cell function. However, the spatial architecture and organization of focal adhesions at a nanometer scale remain unclear. Therefore, we compared the spatial distribution of focal adhesion components within and outside of focal adhesions, using STED microscopy with resolution of 40-50nm. Our results are consistent with the concept that at the nanometer scale, adhesion proteins within but not outside of focal adhesions are composed by nanoscale protein clusters that attach to the extracellular matrix.
Focal adhesions, Nanoscale adhesions, Co-localization, STED microscopy
Physical Sciences Cell Biology
IdentifiersURN: urn:nbn:se:kth:diva-146180OAI: oai:DiVA.org:kth-146180DiVA: diva2:722598
QS 20142014-06-092014-06-092014-06-09Bibliographically approved