Change search
ReferencesLink to record
Permanent link

Direct link
Glycan-Functionalized Fluorescent Chitin Nanocrystals for Biorecognition Applications
KTH, School of Chemical Science and Engineering (CHE), Chemistry.
KTH, School of Chemical Science and Engineering (CHE), Fibre and Polymer Technology.ORCID iD: 0000-0002-4100-6076
KTH, School of Biotechnology (BIO), Glycoscience. KTH, School of Chemical Science and Engineering (CHE), Centres, Wallenberg Wood Science Center.ORCID iD: 0000-0001-9832-027X
Show others and affiliations
2014 (English)In: Bioconjugate chemistry, ISSN 1043-1802, E-ISSN 1520-4812, Vol. 25, no 4, 640-643 p.Article in journal (Refereed) Published
Abstract [en]

A new platform based on chitin nanocrystals has been developed for biorecognition applications. TEMPO-oxidized chitin nanocrystals (TCNs) were labeled with a fluorescent imidazoisoquinolinone dye, and simultaneously conjugated with carbohydrate ligands, resulting in dually functionalized TCNs. The biorecognition properties of the nanocrystals were probed with lectins and bacteria, resulting in selective interactions with their corresponding cognate carbohydrate-binding proteins, as visualized by optical, fluorescence, STEM, and TEM imaging. This represents a new approach to multifunctional nanomaterials based on naturally occurring polymers, holding high potential for biomedical applications.

Place, publisher, year, edition, pages
2014. Vol. 25, no 4, 640-643 p.
Keyword [en]
Concanavalin-A, Soybean Agglutinin, Alpha-Chitin, Lectins, Binding, Nanoparticles, Recognition, Microarray, Proteins, Bacteria
National Category
Biochemistry and Molecular Biology Other Chemistry Topics
URN: urn:nbn:se:kth:diva-145590DOI: 10.1021/bc500004cISI: 000334657500002ScopusID: 2-s2.0-84899004041OAI: diva2:723645
Formas, 2009-1687

QC 20140611

Available from: 2014-06-11 Created: 2014-05-23 Last updated: 2015-08-13Bibliographically approved
In thesis
1. Multifunctional Glyconanomaterials: Applications in Biorecognition and Drug Delivery
Open this publication in new window or tab >>Multifunctional Glyconanomaterials: Applications in Biorecognition and Drug Delivery
2015 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

This thesis consists of three parts, which revolve around the fabrication of multifunctional glyconanomaterials (cellulose nanocrystals, chitin nanocrystals, mesoporous silica nanoparticles) and the development of their applications in biorecognition and nanomedicine.

Part one presents a detailed introduction to glyconanomaterials, focusing on cellulose nanocrystals, chitin nanocrystals and mesoporous silica nanoparticles, including their general preparations, properties and applications.

Part two demonstrates dually modified cellulose and chitin nanocrystals. They are prepared by TEMPO-mediated oxidation, followed by conjugation with a fluorescent dye and carbohydrate ligands. The two functional nanocrystals are applied in carbohydrate-lectin recognition and bacterial imaging as new types of glyconanomaterials.

Part three describes two types of drug delivery systems based on carbohydrate- conjugated mesoporous silica nanoparticles: the first type is a trehalose- functionalized nanoparticle which can selectively recognize mycobacteria, release antimicrobial drugs and kill them; the second type is a lectin-gated drug container, which can perform controlled delivery of drugs to cancer cells in response to glutathione.

Place, publisher, year, edition, pages
Stockholm: KTH Royal Institute of Technology, 2015. 70 p.
TRITA-CHE-Report, ISSN 1654-1081 ; 2015:28
glyconanomaterials, cellulose nanocrystals, chitin nanocrystals, mesoporous silica nanoparticles, lectin, biorecognition, drug delivery, bacteria, cytotoxicity, perfluorophenylazide, fluorescent dye, carbohydrate ligands.
National Category
Nano Technology
urn:nbn:se:kth:diva-171951 (URN)978-91-7595-570-4 (ISBN)
Public defence
2015-09-04, B2, Brinellvägen 23, KTH, Stockholm, 13:00 (English)

QC 20150813

Available from: 2015-08-13 Created: 2015-08-10 Last updated: 2015-08-13Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textScopus

Search in DiVA

By author/editor
Zhou, JuanButchosa, NuriaZhou, QiYan, MingdiRamström, Olof
By organisation
ChemistryFibre and Polymer TechnologyGlycoscienceWallenberg Wood Science CenterOrganic Chemistry
In the same journal
Bioconjugate chemistry
Biochemistry and Molecular BiologyOther Chemistry Topics

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 95 hits
ReferencesLink to record
Permanent link

Direct link