Glycan-Functionalized Fluorescent Chitin Nanocrystals for Biorecognition Applications
2014 (English)In: Bioconjugate chemistry, ISSN 1043-1802, E-ISSN 1520-4812, Vol. 25, no 4, 640-643 p.Article in journal (Refereed) Published
A new platform based on chitin nanocrystals has been developed for biorecognition applications. TEMPO-oxidized chitin nanocrystals (TCNs) were labeled with a fluorescent imidazoisoquinolinone dye, and simultaneously conjugated with carbohydrate ligands, resulting in dually functionalized TCNs. The biorecognition properties of the nanocrystals were probed with lectins and bacteria, resulting in selective interactions with their corresponding cognate carbohydrate-binding proteins, as visualized by optical, fluorescence, STEM, and TEM imaging. This represents a new approach to multifunctional nanomaterials based on naturally occurring polymers, holding high potential for biomedical applications.
Place, publisher, year, edition, pages
2014. Vol. 25, no 4, 640-643 p.
Concanavalin-A, Soybean Agglutinin, Alpha-Chitin, Lectins, Binding, Nanoparticles, Recognition, Microarray, Proteins, Bacteria
Biochemistry and Molecular Biology Other Chemistry Topics
IdentifiersURN: urn:nbn:se:kth:diva-145590DOI: 10.1021/bc500004cISI: 000334657500002ScopusID: 2-s2.0-84899004041OAI: oai:DiVA.org:kth-145590DiVA: diva2:723645
QC 201406112014-06-112014-05-232015-08-13Bibliographically approved