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Understanding the Photochemical Pathway of In Vitro Target Delivery of Aluminium Phthalocyanine: A Mechanistic Approach Using Radical Reaction Chemistry
KTH, School of Engineering Sciences (SCI), Applied Physics.
KTH, School of Biotechnology (BIO), Protein Technology.
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2014 (English)In: ChemPlusChem, ISSN 2192-6506, Vol. 79, no 5, 671-679 p.Article in journal (Refereed) Published
Abstract [en]

A classical dye, aluminium phthalocyanine (AlPc), is used to study the photochemical processes involved in the chromophore-assisted laser inactivation technique. Both cell-free and cell-based systems are investigated by novel methods and radical reaction chemistry. Findings on the photochemical pathways in two models representing cell-free and a cell-based systems are reported. In the cell-free system, the unsubstituted, free, fluorescence-active photosensitiser AlPc recovers its fluorescence signal by means of phosphorescence through a reversible photobleaching process. In the cell-based system, photoactivation of substituted AlPc conjugated to an antibody results in the loss of fluorescence signal at the area examined. Reinjection of the AlPc-conjugated antibodies restores the fluorescence signal.

Place, publisher, year, edition, pages
2014. Vol. 79, no 5, 671-679 p.
Keyword [en]
cleavage reactions, fluorescence, photochemistry, phthalocyanines, radicals
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URN: urn:nbn:se:kth:diva-147048DOI: 10.1002/cplu.201300402ISI: 000335515800005Scopus ID: 2-s2.0-84900405079OAI: oai:DiVA.org:kth-147048DiVA: diva2:728328
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QC 20140624

Available from: 2014-06-24 Created: 2014-06-23 Last updated: 2014-06-24Bibliographically approved

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Brismar, Hjalmar

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