Understanding the Photochemical Pathway of In Vitro Target Delivery of Aluminium Phthalocyanine: A Mechanistic Approach Using Radical Reaction Chemistry
2014 (English)In: ChemPlusChem, ISSN 2192-6506, Vol. 79, no 5, 671-679 p.Article in journal (Refereed) Published
A classical dye, aluminium phthalocyanine (AlPc), is used to study the photochemical processes involved in the chromophore-assisted laser inactivation technique. Both cell-free and cell-based systems are investigated by novel methods and radical reaction chemistry. Findings on the photochemical pathways in two models representing cell-free and a cell-based systems are reported. In the cell-free system, the unsubstituted, free, fluorescence-active photosensitiser AlPc recovers its fluorescence signal by means of phosphorescence through a reversible photobleaching process. In the cell-based system, photoactivation of substituted AlPc conjugated to an antibody results in the loss of fluorescence signal at the area examined. Reinjection of the AlPc-conjugated antibodies restores the fluorescence signal.
Place, publisher, year, edition, pages
2014. Vol. 79, no 5, 671-679 p.
cleavage reactions, fluorescence, photochemistry, phthalocyanines, radicals
Other Chemistry Topics
IdentifiersURN: urn:nbn:se:kth:diva-147048DOI: 10.1002/cplu.201300402ISI: 000335515800005ScopusID: 2-s2.0-84900405079OAI: oai:DiVA.org:kth-147048DiVA: diva2:728328
QC 201406242014-06-242014-06-232014-06-24Bibliographically approved