Mass spectrometric analysis of nanoscale sample volumes extracted from open microchannels after sample preconcentration applied on amyloid beta peptides
2014 (English)In: Analytical and Bioanalytical Chemistry, ISSN 1618-2642, E-ISSN 1618-2650, Vol. 406, no 14, 3521-3524 p.Article in journal (Refereed) Published
A new instrumental concept for extraction of nanovolumes from open microchannels (dimensions 150 mu m x 50 mu m, length 10 mm) manufactured on silicon microchips has been used in combination with a previously developed method for preconcentrating proteins and peptides in the open channels through electromigration. The extracted nanovolumes were further analyzed using nanoelectrospray ionization (nESI) or matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) directly or with subsequent enzymatic protein digestion in a nanodroplet prior to the MS analysis. Preconcentration of the samples resulted in a 15-fold sensitivity increase in nESI for a neurotensin solution, and using MALDI-MS, amyloid beta (A beta) peptides could be detected in concentrations down to 1 nM. The method was also successfully applied for detection of cell culture A beta.
Place, publisher, year, edition, pages
2014. Vol. 406, no 14, 3521-3524 p.
Mass spectrometry, Microchannel, Preconcentration, Amyloid beta, MALDI, Nano-ESI
Biochemistry and Molecular Biology
IdentifiersURN: urn:nbn:se:kth:diva-147047DOI: 10.1007/s00216-014-7781-0ISI: 000336261800030ScopusID: 2-s2.0-84901603229OAI: oai:DiVA.org:kth-147047DiVA: diva2:728330
FunderSwedish Research Council, 621-2009-4095
QC 201406242014-06-242014-06-232016-09-30Bibliographically approved