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GradientOptimizer: An open-source graphical environment for calculating optimized gradients in reversed-phase liquid chromatography
KTH, School of Biotechnology (BIO), Gene Technology. KTH, Centres, Science for Life Laboratory, SciLifeLab. KTH, Centres, SeRC - Swedish e-Science Research Centre.ORCID iD: 0000-0001-5689-9797
2014 (English)In: Proteomics, ISSN 1615-9853, E-ISSN 1615-9861, Vol. 14, no 12, 1464-1466 p.Article in journal (Refereed) Published
Abstract [en]

We here present GradientOptimizer, an intuitive, lightweight graphical user interface to design nonlinear gradients for separation of peptides by reversed-phase liquid chromatography. The software allows to calculate three types of nonlinear gradients, each of them optimizing a certain retention time distribution of interest. GradientOptimizer is straightforward to use, requires minimum processing of the input files, and is supported under Windows, Linux, and OS X platforms. The software is open-source and can be downloaded under an Apache 2.0 license at https://github.com/statisticalbiotechnology/NonlinearGradientsUI.

Place, publisher, year, edition, pages
2014. Vol. 14, no 12, 1464-1466 p.
Keyword [en]
Bioinformatics, Chromatography, Retention time, RPLC
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:kth:diva-147957DOI: 10.1002/pmic.201400036ISI: 000337616100003Scopus ID: 2-s2.0-84902208637OAI: oai:DiVA.org:kth-147957DiVA: diva2:733757
Funder
Science for Life Laboratory - a national resource center for high-throughput molecular bioscience
Note

QC 20140711

Available from: 2014-07-11 Created: 2014-07-10 Last updated: 2017-12-05Bibliographically approved

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Käll, Lukas

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