Scanning inverse fluorescence correlation spectroscopy
2014 (English)In: Optics Express, ISSN 1094-4087, E-ISSN 1094-4087, Vol. 22, no 11, 13073-13090 p.Article in journal (Refereed) Published
Scanning Inverse Fluorescence Correlation Spectroscopy (siFCS) is introduced to determine the absolute size of nanodomains on surfaces. We describe here equations for obtaining the domain size from cross-and auto-correlation functions, measurement simulations which enabled testing of these equations, and measurements on model surfaces mimicking membranes containing nanodomains. Using a confocal microscope of 270 nm resolution the size of 250 nm domains were estimated by siFCS to 257 +/- 12 nm diameter, and 40 nm domains were estimated to 65 +/- 26 nm diameter. Applications of siFCS for sizing of nanodomains and protein clusters in cell membranes are discussed.
Place, publisher, year, edition, pages
Optical Society of America, 2014. Vol. 22, no 11, 13073-13090 p.
Image Correlation Spectroscopy, Cross-Correlation Spectroscopy, Stimulated-Emission, Microscopy, Nanoscopy, Proteins, Cells, Limit
Research subject Biological Physics
IdentifiersURN: urn:nbn:se:kth:diva-148302DOI: 10.1364/OE.22.013073ISI: 000337501600037ScopusID: 2-s2.0-84901830506OAI: oai:DiVA.org:kth-148302DiVA: diva2:736674
FunderScience for Life Laboratory - a national resource center for high-throughput molecular bioscience
QC 201408082014-08-082014-08-052016-03-10Bibliographically approved