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Scanning inverse fluorescence correlation spectroscopy
KTH, School of Engineering Sciences (SCI), Applied Physics, Experimental Biomolecular Physics.
KTH, School of Engineering Sciences (SCI), Applied Physics, Experimental Biomolecular Physics.
KTH, School of Engineering Sciences (SCI), Applied Physics, Experimental Biomolecular Physics.ORCID iD: 0000-0003-3200-0374
KTH, School of Engineering Sciences (SCI), Applied Physics, Experimental Biomolecular Physics. KTH, Centres, Science for Life Laboratory, SciLifeLab.ORCID iD: 0000-0002-1850-5440
2014 (English)In: Optics Express, ISSN 1094-4087, E-ISSN 1094-4087, Vol. 22, no 11, 13073-13090 p.Article in journal (Refereed) Published
Abstract [en]

Scanning Inverse Fluorescence Correlation Spectroscopy (siFCS) is introduced to determine the absolute size of nanodomains on surfaces. We describe here equations for obtaining the domain size from cross-and auto-correlation functions, measurement simulations which enabled testing of these equations, and measurements on model surfaces mimicking membranes containing nanodomains. Using a confocal microscope of 270 nm resolution the size of 250 nm domains were estimated by siFCS to 257 +/- 12 nm diameter, and 40 nm domains were estimated to 65 +/- 26 nm diameter. Applications of siFCS for sizing of nanodomains and protein clusters in cell membranes are discussed.

Place, publisher, year, edition, pages
Optical Society of America, 2014. Vol. 22, no 11, 13073-13090 p.
Keyword [en]
Image Correlation Spectroscopy, Cross-Correlation Spectroscopy, Stimulated-Emission, Microscopy, Nanoscopy, Proteins, Cells, Limit
National Category
Biophysics
Research subject
Biological Physics
Identifiers
URN: urn:nbn:se:kth:diva-148302DOI: 10.1364/OE.22.013073ISI: 000337501600037Scopus ID: 2-s2.0-84901830506OAI: oai:DiVA.org:kth-148302DiVA: diva2:736674
Funder
Science for Life Laboratory - a national resource center for high-throughput molecular bioscience
Note

QC 20140808

Available from: 2014-08-08 Created: 2014-08-05 Last updated: 2017-12-05Bibliographically approved

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Bergstrand et al Opt Expr 2014(892 kB)51 downloads
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Wennmalm, Stefan

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