Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
A biomimicking approach for hemicellulose processing
KTH, School of Chemical Science and Engineering (CHE), Fibre and Polymer Technology, Wood Chemistry and Pulp Technology.
2014 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Lignocellulose can become the best opportunity for the society to reduce its dependency on the harmful petroleum based products as well as to produce clean energy. In each part of the production cycle, biomass based products have a better environmental profiles than their petroleum based counterparts. Woody biomass has a vast availability, but it suffers from recalcitrance that is mostly caused by lignin that is functioning as a matrix, surrounding and binding the carbohydrates that are currently the most valuable of the wood components.

Lignin-carbohydrate (LC) bonds are believed to be a key element in this recalcitrance and research has shown that these types of bonds are common in wood. These bonds are important in an economical point of view as well, as e.g. residual lignin structures in pulp (lignins bonded to the cellulose and hemicelluloses) require expensive bleaching sequences for their removal.

The LC-structures can also be exploited technically as we now have demonstrated. We developed a method that utilizes phenolic end groups that are bonded to different hemicelluloses for cross-linking. The enzyme laccase was used for the cross-linking to create a cost-efficient processing scheme to both isolate and increase the molecular weight of the hemicelluloses. Membrane filtration was used as the key separation technique, which enables the establishment of industrial scale production. The final product had improved mechanical and thermal properties and could be used e.g. as barrier film component in renewable packaging. Nanocomposite formation with nanofibrillated cellulose was also studied. This improved the film properties further. The complexes are also possible to use as model compounds for lignin-carbohydrate complexes in wood.

This technique can also be seen to mimick the lignification and lignin-carbohydrate network formation phenomena in plants enabling the formation of entire networks of wood components. Our results suggests that the side chains of hemicellulose might play an important role in network formation and that hemicellulose molecules can carry more than one lignin phenolic end group to fulfill this capability.

Place, publisher, year, edition, pages
Stockholm: KTH Royal Institute of Technology, 2014. , 50 p.
Series
TRITA-CHE-Report, ISSN 1654-1081 ; 2014:30
Keyword [en]
Mechanical pulping, Hemicellulose, Cross-linking, Lignin-carbohydrate-complex
National Category
Polymer Technologies
Research subject
Fibre and Polymer Science
Identifiers
URN: urn:nbn:se:kth:diva-148586ISBN: 978-91-7595-221-5 (print)OAI: oai:DiVA.org:kth-148586DiVA: diva2:736798
Public defence
2014-09-05, Kollegiesalen, Brinellvägen 8, KTH, Stockholm, 13:00 (English)
Opponent
Supervisors
Funder
Swedish Research Council, 621-2008-4177Knut and Alice Wallenberg FoundationVinnova, 2011-03387
Note

QC 20140825

Available from: 2014-08-25 Created: 2014-08-08 Last updated: 2014-08-25Bibliographically approved
List of papers
1. Enzyme catalyzed cross-linking of spruce galactoglucomannan improves its applicability in barrier films
Open this publication in new window or tab >>Enzyme catalyzed cross-linking of spruce galactoglucomannan improves its applicability in barrier films
2013 (English)In: Carbohydrate Polymers, ISSN 0144-8617, E-ISSN 1879-1344, Vol. 95, no 2, 690-696 p.Article in journal (Refereed) Published
Abstract [en]

Hemicelluloses are one of the main constituents of plant cell walls and thereby one of the most abundant biopolymers on earth. They can be obtained as by-products from different wood based processes, most importantly from the mechanical pulping. Hemicelluloses have interesting properties in e.g. barrier film applications. However, their relatively low molecular weight after isolation and co-extraction with lignin has limited their use. In this work, we present a novel technique for increasing the molecular weight of different wood hemicelluloses from mechanical pulping process waters as well as from pre-hydrolysis extracts. This is achieved by enzyme-catalyzed cross-linking of aromatic moieties bound to the hemicelluloses. The cross-linking treatment resulted in significantly improved mechanical properties in barrier films made with spruce galactoglucomannan. To our knowledge, this is the first time that wood hemicelluloses have been cross-linked by utilizing the bound aromatic moieties and creates new possibilities for utilizing this raw material source.

Keyword
Hemicellulose, Laccase, Cross-linking, Barrier film, Mechanical pulping
National Category
Polymer Chemistry
Identifiers
urn:nbn:se:kth:diva-124444 (URN)10.1016/j.carbpol.2013.03.016 (DOI)000319644900012 ()2-s2.0-84877578791 (Scopus ID)
Funder
Swedish Research Council, 621-2008-4177
Note

QC 20130710

Available from: 2013-07-10 Created: 2013-07-05 Last updated: 2017-12-06Bibliographically approved
2. On the formation of lignin polysaccharide networks in Norway spruce
Open this publication in new window or tab >>On the formation of lignin polysaccharide networks in Norway spruce
2015 (English)In: Phytochemistry, ISSN 0031-9422, E-ISSN 1873-3700, Vol. 111, 177-184 p.Article in journal (Refereed) Published
Abstract [en]

In this study we were mirroring suggested in vivo phenomena of lignin-hemicellulose complex formation in vitro, by cross-linking Norway spruce (Picea abies) galactoglucomannans, xylans and lignin moieties to high molecular weight complexes by laccase treatment. We were able to observe the oxidation and cross-linking of non-condensed guaiacyl-type phenolic moieties attached to both of the hemicelluloses by P-31 NMR and size-exclusion chromatography. We suggest that hemicelluloses-lignin complexes form covalently linked structural units during the early stages of lignification via radical enzymatic cross-linking catalyzed by laccase. This work shows that the hemicellulose molecules in wood are covalently linked to two or more lignin units thereby making them suited for forming network structures.

Keyword
Norway spruce, Galactoglucomannan, Arabinoxylan, Lignin-carbohydrate networks, Laccase, Crosslinking, Lignification
National Category
Biochemistry and Molecular Biology
Identifiers
urn:nbn:se:kth:diva-149640 (URN)10.1016/j.phytochem.2014.10.027 (DOI)000350527400022 ()25549980 (PubMedID)2-s2.0-84922731900 (Scopus ID)
Funder
Swedish Research Council, 621-2008-4177VINNOVA, 2011-03387
Note

QC 20150408. Updated from manuscript to article in journal.

Available from: 2014-08-25 Created: 2014-08-25 Last updated: 2017-12-05Bibliographically approved
3. A method for studying effects on lignin-polysaccharide networks during degradation and technical processing of wood
Open this publication in new window or tab >>A method for studying effects on lignin-polysaccharide networks during degradation and technical processing of wood
(English)Manuscript (preprint) (Other academic)
Identifiers
urn:nbn:se:kth:diva-149642 (URN)
Note

QS 2014

Available from: 2014-08-25 Created: 2014-08-25 Last updated: 2014-09-03Bibliographically approved
4. Structural studies of an enzymatically produced Norway spruce lignin-carbohydrate complex
Open this publication in new window or tab >>Structural studies of an enzymatically produced Norway spruce lignin-carbohydrate complex
(English)Manuscript (preprint) (Other academic)
Identifiers
urn:nbn:se:kth:diva-149643 (URN)
Note

QS 2014

Available from: 2014-08-25 Created: 2014-08-25 Last updated: 2014-08-25Bibliographically approved
5. The composite formation of cross-linked galactoglucomannan-lignin networks and cellulose nanoparticles as defined by thermal and mechanical testing
Open this publication in new window or tab >>The composite formation of cross-linked galactoglucomannan-lignin networks and cellulose nanoparticles as defined by thermal and mechanical testing
Show others...
(English)Manuscript (preprint) (Other academic)
Identifiers
urn:nbn:se:kth:diva-149645 (URN)
Note

QS 2014

Available from: 2014-08-25 Created: 2014-08-25 Last updated: 2014-08-25Bibliographically approved
6. Combined membrane filtration and enzymatic treatment for recovery of high molecular mass hemicelluloses from chemithermomechanical pulp process water
Open this publication in new window or tab >>Combined membrane filtration and enzymatic treatment for recovery of high molecular mass hemicelluloses from chemithermomechanical pulp process water
2013 (English)In: Chemical Engineering Journal, ISSN 1385-8947, E-ISSN 1873-3212, Vol. 225, 292-299 p.Article in journal (Refereed) Published
Abstract [en]

Hemicelluloses with high molecular mass are needed for the manufacture of value added products such as food packaging barrier films. In this work such molecules were recovered from chemithermomechanical pulp (CTMP) process water using an innovative three-stage process comprising membrane separation and enzymatic treatment with laccase. Microfiltration followed by ultrafiltration was found to be a suitable combination in the first stage, providing a concentrated and purified hemicellulose fraction suitable for enzymatic treatment. In both membrane processes a high average flux (260 and 115 l/m(2) h) and a low fouling tendency were observed. A marked increase in the average molecular mass of hemicelluloses with bound lignin moieties was achieved by laccase treatment in the second stage. The enzymatically crosslinked hemicelluloses were finally recovered in the third stage using ultrafiltration. In the final high molecular mass solution the hemicellulose concentration was 54 g/l, the contribution of hemicelluloses to the total solids content 43%, and the viscosity of the solution 27 mPa s. The results demonstrate that a hemicellulose fraction of high quality can be produced from CTMP process water, and that this could constitute a suitable feedstock for the production of, for example, barrier films for renewable packaging.

Place, publisher, year, edition, pages
Elsevier, 2013
Keyword
Chemithermomechanical pulp, Hemicellulose, Galactoglucomannan, Membrane filtration, Laccase
National Category
Chemical Engineering
Identifiers
urn:nbn:se:kth:diva-125753 (URN)10.1016/j.cej.2013.03.089 (DOI)000321313800034 ()2-s2.0-84876733717 (Scopus ID)
Funder
Swedish Research CouncilVinnova
Note

QC 20130813

Available from: 2013-08-13 Created: 2013-08-13 Last updated: 2017-12-06Bibliographically approved

Open Access in DiVA

Doctoral thesis Petri Oinonen(5005 kB)552 downloads
File information
File name FULLTEXT01.pdfFile size 5005 kBChecksum SHA-512
90f1667e4a3bc7d638daee16ba60a783e073d776c26af6fb2a3e4f67ce192497e0521e04ed01e848a153966222c71c9c4a3e1b68fdb540792426686f952d447e
Type fulltextMimetype application/pdf

Search in DiVA

By author/editor
Oinonen, Petri
By organisation
Wood Chemistry and Pulp Technology
Polymer Technologies

Search outside of DiVA

GoogleGoogle Scholar
Total: 552 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

isbn
urn-nbn

Altmetric score

isbn
urn-nbn
Total: 1135 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf