Deterministic lateral displacement device for droplet separation by size - Towards rapid clonal selection based on droplet shrinking
2010 (English)In: 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010: Volume 2, 2010, 1355-1357 p.Conference paper (Refereed)
We present a novel method for robust passive separation of microfluidic droplets by size using deterministic lateral displacement(DLD). We also show that droplets containing Saccharomyces Cervisiae shrink significantly during incubation while droplets containing only yeast media retain their size. We demonstrate the DLD device by sorting out shrunken yeast-cell containing droplets from a 40-fold excess of ∼33% larger yeast-cell-free droplets generated at the same time, suggesting that DLD might be used for clonal selection. The same device also separates 11 μm from 30μm droplets at a rate of 12000droplets/second, more than twofold faster than previously demonstrated passive hydrodynamic separation devices .
Place, publisher, year, edition, pages
2010. 1355-1357 p.
Cells, Droplets, High-Throughput, Separation
IdentifiersURN: urn:nbn:se:kth:diva-148864ScopusID: 2-s2.0-84884381984ISBN: 978-161839062-2OAI: oai:DiVA.org:kth-148864DiVA: diva2:738112
14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010, 3 October 2010 through 7 October 2010, Groningen, Netherlands
QC 201408152014-08-152014-08-142014-08-15Bibliographically approved