Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Deterministic lateral displacement device for droplet separation by size - Towards rapid clonal selection based on droplet shrinking
KTH, School of Biotechnology (BIO), Nano Biotechnology (closed 20130101).ORCID iD: 0000-0001-5232-0805
KTH, School of Biotechnology (BIO), Proteomics (closed 20130101).ORCID iD: 0000-0001-8993-048X
KTH, School of Biotechnology (BIO), Nano Biotechnology (closed 20130101).
2010 (English)In: 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010: Volume 2, 2010, 1355-1357 p.Conference paper, Published paper (Refereed)
Abstract [en]

We present a novel method for robust passive separation of microfluidic droplets by size using deterministic lateral displacement(DLD). We also show that droplets containing Saccharomyces Cervisiae shrink significantly during incubation while droplets containing only yeast media retain their size. We demonstrate the DLD device by sorting out shrunken yeast-cell containing droplets from a 40-fold excess of ∼33% larger yeast-cell-free droplets generated at the same time, suggesting that DLD might be used for clonal selection. The same device also separates 11 μm from 30μm droplets at a rate of 12000droplets/second, more than twofold faster than previously demonstrated passive hydrodynamic separation devices [1].

Place, publisher, year, edition, pages
2010. 1355-1357 p.
Keyword [en]
Cells, Droplets, High-Throughput, Separation
National Category
Industrial Biotechnology
Identifiers
URN: urn:nbn:se:kth:diva-148864Scopus ID: 2-s2.0-84884381984ISBN: 978-161839062-2 (print)OAI: oai:DiVA.org:kth-148864DiVA: diva2:738112
Conference
14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010, 3 October 2010 through 7 October 2010, Groningen, Netherlands
Funder
Vinnova
Note

QC 20140815

Available from: 2014-08-15 Created: 2014-08-14 Last updated: 2014-08-15Bibliographically approved

Open Access in DiVA

No full text

Scopus

Authority records BETA

Jönsson, HåkanUhlén, Mathias

Search in DiVA

By author/editor
Jönsson, HåkanUhlén, MathiasAndersson-Svahn, Helene
By organisation
Nano Biotechnology (closed 20130101)Proteomics (closed 20130101)
Industrial Biotechnology

Search outside of DiVA

GoogleGoogle Scholar

isbn
urn-nbn

Altmetric score

isbn
urn-nbn
Total: 23 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf