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A homogeneous assay for biomolecule interaction analysis in droplets by flourescence polarization
KTH, School of Biotechnology (BIO), Nano Biotechnology (closed 20130101).ORCID iD: 0000-0001-5232-0805
KTH, School of Biotechnology (BIO), Nano Biotechnology (closed 20130101).
KTH, School of Biotechnology (BIO), Proteomics (closed 20130101).ORCID iD: 0000-0001-8993-048X
KTH, School of Biotechnology (BIO), Proteomics (closed 20130101).
2010 (English)In: 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010: Volume 3, 2010, 1802-1804 p.Conference paper, Published paper (Refereed)
Abstract [en]

We present a novel homogeneous assay for detecting biomolecule interactions in microdroplets by fluorescence polarization (FP) for the first time. The FP assay allows the detection of target biomolecules directly after incubation without removing the detection reagent by separation or washing, making the assay amenable to automation. Using this assay we evaluate protein-protein and drug-DNA interactions. We detect these interactions at concentrations as low as 100nM and 69 pM respectively. This is a proof-of-concept homogeneous labeling assay in droplets for detecting bio-macromolecules.

Place, publisher, year, edition, pages
2010. 1802-1804 p.
Keyword [en]
Biomolecule, Fluorescence polarization, Homogeneous assay, Microdroplets
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:kth:diva-148930Scopus ID: 2-s2.0-84884321517ISBN: 978-161839062-2 (print)OAI: oai:DiVA.org:kth-148930DiVA: diva2:738140
Conference
14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010, 3 October 2010 through 7 October 2010, Groningen, Netherlands
Note

QC 20140815

Available from: 2014-08-15 Created: 2014-08-14 Last updated: 2014-08-15Bibliographically approved

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Jönsson, HåkanUhlén, Mathias

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Jönsson, HåkanZhang, ChiUhlén, MathiasAndersson Svahn, Helene
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Output format
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