A homogeneous assay for biomolecule interaction analysis in droplets by flourescence polarization
2010 (English)In: 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010: Volume 3, 2010, 1802-1804 p.Conference paper (Refereed)
We present a novel homogeneous assay for detecting biomolecule interactions in microdroplets by fluorescence polarization (FP) for the first time. The FP assay allows the detection of target biomolecules directly after incubation without removing the detection reagent by separation or washing, making the assay amenable to automation. Using this assay we evaluate protein-protein and drug-DNA interactions. We detect these interactions at concentrations as low as 100nM and 69 pM respectively. This is a proof-of-concept homogeneous labeling assay in droplets for detecting bio-macromolecules.
Place, publisher, year, edition, pages
2010. 1802-1804 p.
Biomolecule, Fluorescence polarization, Homogeneous assay, Microdroplets
IdentifiersURN: urn:nbn:se:kth:diva-148930ScopusID: 2-s2.0-84884321517ISBN: 978-161839062-2OAI: oai:DiVA.org:kth-148930DiVA: diva2:738140
14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010, 3 October 2010 through 7 October 2010, Groningen, Netherlands
QC 201408152014-08-152014-08-142014-08-15Bibliographically approved