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Towards automated high content screening on a 672-microwell slide
KTH, School of Biotechnology (BIO), Nano Biotechnology (closed 20130101).
KTH, School of Biotechnology (BIO), Nano Biotechnology (closed 20130101).
2010 (English)In: 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010: Volume 2, 2010, 965-967 p.Conference paper, Published paper (Refereed)
Abstract [en]

We present an automated high content screening method for single-cell analysis on a microwell slide. The 672 wells are screened in 73 minutes, i.e. a 10-fold increase, yielding a negative/positive response using an automated confocal software. This screening method is highly sophisticated, rapid and results in high-resolution imaging. The next step is to apply the method to a heterogeneous glioma cell line using a stem cell- and astrocyte marker to study the link between marker expression and proliferation rate on single cells. The resulting images are input files in a cell analyzing software to attain information about cell density, co-localization, cell size and cell count.

Place, publisher, year, edition, pages
2010. 965-967 p.
Keyword [en]
Automated Cell Analysis, High Content Screening, Microwell Slide, Single-Cell
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:kth:diva-148883Scopus ID: 2-s2.0-84884404701ISBN: 978-161839062-2 (print)OAI: oai:DiVA.org:kth-148883DiVA: diva2:738305
Conference
14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010, 3 October 2010 through 7 October 2010, Groningen, Netherlands
Note

QC 20140818

Available from: 2014-08-18 Created: 2014-08-14 Last updated: 2014-08-18Bibliographically approved

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  • apa
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  • nn-NB
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  • Other locale
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Output format
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