On the formation of lignin polysaccharide networks in Norway spruce
2015 (English)In: Phytochemistry, ISSN 0031-9422, E-ISSN 1873-3700, Vol. 111, 177-184 p.Article in journal (Refereed) Published
In this study we were mirroring suggested in vivo phenomena of lignin-hemicellulose complex formation in vitro, by cross-linking Norway spruce (Picea abies) galactoglucomannans, xylans and lignin moieties to high molecular weight complexes by laccase treatment. We were able to observe the oxidation and cross-linking of non-condensed guaiacyl-type phenolic moieties attached to both of the hemicelluloses by P-31 NMR and size-exclusion chromatography. We suggest that hemicelluloses-lignin complexes form covalently linked structural units during the early stages of lignification via radical enzymatic cross-linking catalyzed by laccase. This work shows that the hemicellulose molecules in wood are covalently linked to two or more lignin units thereby making them suited for forming network structures.
Place, publisher, year, edition, pages
2015. Vol. 111, 177-184 p.
Norway spruce, Galactoglucomannan, Arabinoxylan, Lignin-carbohydrate networks, Laccase, Crosslinking, Lignification
Biochemistry and Molecular Biology
IdentifiersURN: urn:nbn:se:kth:diva-149640DOI: 10.1016/j.phytochem.2014.10.027ISI: 000350527400022PubMedID: 25549980ScopusID: 2-s2.0-84922731900OAI: oai:DiVA.org:kth-149640DiVA: diva2:740449
FunderSwedish Research Council, 621-2008-4177VINNOVA, 2011-03387
QC 20150408. Updated from manuscript to article in journal.2014-08-252014-08-252015-04-08Bibliographically approved