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A ten-minute high density lateral flow protein microarray assay
KTH, School of Biotechnology (BIO), Nano Biotechnology (closed 20130101).
KTH, School of Biotechnology (BIO), Molecular Biotechnology (closed 20130101).ORCID iD: 0000-0003-1598-8894
KTH, School of Biotechnology (BIO), Proteomics (closed 20130101).
KTH, School of Biotechnology (BIO), Proteomics (closed 20130101).
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2011 (English)In: 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011: (MicroTAS 2011), 2011, 1176-1178 p.Conference paper, Published paper (Refereed)
Abstract [en]

Protein microarrays are useful tools for highly multiplexed determination of presence or levels of clinically relevant biomarkers in human tissues and biofluids. However, such tools have thus far been restricted to laboratory environments. Here, we present a novel 384-plexed easy to use lateral flow protein microarray device capable of sensitive (<50ng/ml) determination of antigen specific antibodies in less than ten minutes total assay time. Results were developed with gold nanobeads and could be recorded by a cell-phone camera or table top scanner. Excellent accuracy (AUC=99.4%) was achieved in comparison with an established glass microarray assay for 26 antigen-specific antibodies.

Place, publisher, year, edition, pages
2011. 1176-1178 p.
Keyword [en]
Lateral flow, Point of care, Protein microarray, Biofluids, Cell phone camera, Human tissues, Laboratory environment, Nanobeads, Antibodies, Antigens, Bioassay, Biochips, Tools
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:kth:diva-148732Scopus ID: 2-s2.0-84883789603ISBN: 9781618395955 (print)OAI: oai:DiVA.org:kth-148732DiVA: diva2:740699
Conference
15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011, 2 October 2011 through 6 October 2011, Seattle, WA
Note

QC 20140826

Available from: 2014-08-26 Created: 2014-08-11 Last updated: 2014-08-26Bibliographically approved

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Bass, Tarek

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Gantelius, JesperBass, TarekGundberg, AnnaSundberg, MårtenSjöberg, RonaldNilsson, PeterAndersson-Svahn, Helene
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Nano Biotechnology (closed 20130101)Molecular Biotechnology (closed 20130101)Proteomics (closed 20130101)
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CiteExportLink to record
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Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
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More styles
Language
  • de-DE
  • en-GB
  • en-US
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  • nn-NO
  • nn-NB
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  • Other locale
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Output format
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  • asciidoc
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