Tissue-specific protein expression in human cells, tissues and organs
2010 (English)In: Journal of Proteomics & Bioinformatics, ISSN 0974-276X, Vol. 3, no 10, 286-293 p.Article in journal (Refereed) Published
An important part of understanding human biology is the study of tissue-specific expression both at the gene and protein level. In this study, the analysis of tissue specific protein expression was performed based on tissue micro array data available on the public Human Protein Atlas database (www.proteinatlas.org). An analysis of human proteins, corresponding to approximately one third of the protein-encoding genes, was carried out in 65 human tissues and cell types. The spatial distribution and relative abundance of 6,678 human proteins, were analyzed in different cell populations from various organs and tissues in the human body using unsupervised methods, such as hierarchical clustering and principal component analysis, as well as with supervised methods (Breiman, 2001). Well-known markers, such as neuromodulin for the central nervous system, keratin 20 for gastrointestinal tract and CD45 for hematopoietic cells, were identified as tissue-specific. Proteins expressed in a tissue-specific manner were identified for cells in all of the investigated tissues, including the central nervous system, hematopoietic system, squamous epithelium, mesenchymal cells and cells from the gastrointestinal tract. Several proteins not yet associated with tissue-specificity were identified, providing starting points for further studies to explore tissue-specific functions. This includes proteins with no known function, such as ZNF509 expressed in CNS and C1orf201 expressed in the gastro-intestinal tract. In general, the majority of the gene products are expressed in a ubiquitous manner and few proteins are detected exclusively in cells from a particular tissue class, as exemplified by less than 1% of the analyzed proteins found only in the brain.
Place, publisher, year, edition, pages
2010. Vol. 3, no 10, 286-293 p.
C1orf201 protein, cadherin 17, CD45 antigen, cytokeratin 1, cytokeratin 14, cytokeratin 20, cytokeratin 36, desmin, ELAVL4 proten, emelin, excitatory amino acid transporter 1, neuromodulin, protein, unclassified drug, ZNF509 protein
Bioinformatics and Systems Biology
IdentifiersURN: urn:nbn:se:kth:diva-149700DOI: 10.4172/jpb.1000153ScopusID: 2-s2.0-78249263183OAI: oai:DiVA.org:kth-149700DiVA: diva2:741301
FunderScience for Life Laboratory - a national resource center for high-throughput molecular bioscience
QC 201408272014-08-272014-08-262014-08-27Bibliographically approved