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Morphological analysis of articular cartilage using multiphoton microscopy as input for constitutive modeling: Experiment and mathematical implementation
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2010 (English)In: 6th World Congress Of Biomechanics (WCB 2010), Pts 1-3, Springer-Verlag New York, 2010, 895-898 p.Conference paper (Refereed)
Abstract [en]

The 3D structure of collagen fibers in chicken cartilage was quantified using multiphoton microscopy. Samples of fresh chicken cartilage were sectioned in three orthogonal planes using a vibratome. The sections were imaged using multiphoton microscopy, specifically imaging the collagen fibers using the second harmonic signal. Employing image analysis techniques based on Fourier analysis, the primary direction and anisotropy of the collagen fibers were extracted for the superficial layer resulting in a 3D map of the collagen fiber fabric. In the middle layer, image analysis using objective thresholding techniques was employed to extract the volume fraction occupied by extracellular matrix, the rest being occupied by the lacunae and residing chondrocytes. To implement these imaging data in a computational setting, we propose a new, 3D large strain constitutive model for articular cartilage, focused on the essential load-bearing morphology: an inhomogeneous, visco-poroelastic solid matrix reinforced by an anisotropic, viscoelastic dispersed fiber fabric which is saturated by an incompressible fluid residing in strain-dependent pores of the collagen-proteoglycan solid matrix. High-fidelity models, combining advanced imaging and computational biomechanics, will allow us to consider complex problems in structure-function relationships and provide insight to microphysical (mechanobiological) cellular stimuli.

Place, publisher, year, edition, pages
Springer-Verlag New York, 2010. 895-898 p.
, IFMBE Proceedings, ISSN 1680-0737 ; 31 IFMBE
Keyword [en]
Articular cartilage, Constitutive modeling, Finite element simulation, Multiphoton microscopy imaging
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Other Biological Topics
URN: urn:nbn:se:kth:diva-149721DOI: 10.1007/978-3-642-14515-5_228ISI: 000306716100228ScopusID: 2-s2.0-77958018219ISBN: 978-3-642-14514-8OAI: diva2:741521
6th World Congress of Biomechanics, WCB 2010 - In Conjunction with 14th International Conference on Biomedical Engineering, ICBME and 5th Asia Pacific Conference on Biomechanics, APBiomech, 1 August 2010 through 6 August 2010, Singapore

QC 20140828

Available from: 2014-08-28 Created: 2014-08-26 Last updated: 2014-08-28Bibliographically approved

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Holzapfel, Gerhard A.
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