Hydrolysis of RNA by a lanthanide salen - cyclodextrin complex
Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesis
This is a report focusing on further development of a synthetic catalyst for hydrolysis of the phosphate ester bonds in RNA. The main goal was to construct a catalyst based on a cyclodextrin ligand suitable for carrying a lanthanide metal with the induced capability to hydrolyse the stable phosphate ester bonds in RNA. This was achieved through a six-step synthesis resulting in a lanthanide-salen-cyclodextrin complex. For simplicity in the product analysis of the reaction, adenosine diribonucleotide (ApA) was used as a model substrate for RNA. The hydrolysis was performed at 20°C and ph7 to mimic physiological conditions in the human body. The results showed indications that it was possible to hydrolysis RNA by this kind of complex.
Place, publisher, year, edition, pages
Engineering and Technology
IdentifiersURN: urn:nbn:se:kth:diva-149649OAI: oai:DiVA.org:kth-149649DiVA: diva2:744695